Tian Fang, Wang Yongbo, Guo Guang, Ding Keqiang, Yang Feng, Wang Chongyang, Wang Huiya, Yan Mengdi
College of Environmental Engineering, Nanjing Institute of Technology, Nanjing, 211167, China.
Shijiazhuang Institute of Pomology, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang, 050061, China.
Environ Res. 2023 Nov 15;237(Pt 1):116828. doi: 10.1016/j.envres.2023.116828. Epub 2023 Aug 7.
Treating textile wastewaters were always inhibited by its higher salt concentration and temperature. In this study, a halo-thermophilic bacterial consortium YM was enriched with ability to decolorize acid brilliant scarlet GR (ABS) at 55 °C and 10% salinity. Under optimum conditions of pH (8), temperature (55 °C), and salinity (10%), YM decolorized 97% of ABS under anaerobic conditions. Alteribacillus was identified to be the dominant genus in consortium YM. Consortium YM showed significant decolorization ability under a wide range of salinity (1%-10%), pH (7-9) and temperature (45 °C-60 °C). The degradation pathway of ABS was proposed by the combination of UV-vis spectral analysis, Fourier transform infrared (FTIR), gas chromatography mass spectrometric (GC-MS), and metagenomic analysis. Azoreductase, which was an important enzyme in decolorization process, was identified with great variation in the genome of consortium YM. Meanwhile, the metabolic intermediates after decolorization was identified with low biotoxicity by phytotoxicity tests. This study first identified that Alterbacillus play an important role in azo dye decolorization and degradation process under halo-thermophlic conditions and provided significant knowledge for azo dye decolorization and degradation process.
纺织废水的处理一直受到其高盐浓度和温度的抑制。在本研究中，富集了一个嗜盐嗜热细菌群落YM，其能够在55℃和10%盐度下对偶氮染料酸性艳红GR（ABS）进行脱色。在pH值为8、温度为55℃、盐度为10%的最佳条件下，YM在厌氧条件下对偶氮染料酸性艳红GR的脱色率达到97%。已鉴定出Alteribacillus是群落YM中的优势属。群落YM在较宽的盐度范围（1%-10%）、pH值范围（7-9）和温度范围（45℃-60℃）内均表现出显著的脱色能力。通过紫外可见光谱分析、傅里叶变换红外光谱（FTIR）、气相色谱-质谱联用（GC-MS）和宏基因组分析相结合的方法，推测了酸性艳红GR的降解途径。偶氮还原酶是脱色过程中的一种重要酶，在群落YM的基因组中发现其存在很大差异。同时，通过植物毒性试验确定了脱色后的代谢中间体具有低生物毒性。本研究首次确定了Alterbacillus在嗜盐嗜热条件下对偶氮染料的脱色和降解过程中发挥重要作用，并为偶氮染料的脱色和降解过程提供了重要知识。
