Wang Wenying, Zhang Zuotao, Sun Meichen, Li Chenlai, Yan Mengdi, Wang Chongyang
Miami College, Henan University, Kaifeng, 475000, Henan, China.
Department of Environmental Science and Engineering, University of Science and Technology of China, Hefei, 230026, China.
Extremophiles. 2024 Dec 23;29(1):11. doi: 10.1007/s00792-024-01376-w.
Azo dye wastewater has garnered significant attention from researchers because of its association with high-temperature, high-salt, and high-alkali conditions. In this study, consortium ZZ efficiently decolorized brown D3G under halophilic and thermophilic conditions. he results indicated that consortium ZZ, which was mainly dominated by Marinobacter, Bacillus, and Halomonas, was achieved decolorization rates ranging from 1 to 10% at temperatures between 40 °C and 50 °C, while maintaining a pH range of 7 to 10 for direct brown D3G degradation. Through the comprehensive utilization of UV-vis spectral analysis, Fourier transform infrared (FTIR), gas chromatography mass spectrometric (GC-MS) techniques, as well as metagenomic analysis, the decolorization and degradation pathway of direct brown by consortium ZZ was proposed. The azo dye reductase, lignin peroxidase, and laccase were also highly expressed in the decolorization process. Additionally, phytotoxicity tests using seeds of Cucumis sativus and Oryza sativa revealed that the intermediates generated showed no significant toxicity compared with distilled water. This investigation elucidated the pivotal contribution of consortium ZZ to azo dye degradation and provided novel theoretical insights along with practical guidance for azo dye treatment at halo-thermophilic conditions.
偶氮染料废水因其与高温、高盐和高碱条件相关而受到研究人员的广泛关注。在本研究中，联合体ZZ在嗜盐和嗜热条件下能有效地使直接棕D3G脱色。结果表明，以海杆菌属、芽孢杆菌属和嗜盐单胞菌属为主的联合体ZZ在40℃至50℃的温度下实现了1%至10%的脱色率，同时在pH值为7至10的范围内对直接棕D3G进行降解。通过综合利用紫外可见光谱分析、傅里叶变换红外光谱（FTIR）、气相色谱 - 质谱联用（GC - MS）技术以及宏基因组分析，提出了联合体ZZ对直接棕的脱色和降解途径。偶氮染料还原酶、木质素过氧化物酶和漆酶在脱色过程中也高度表达。此外，使用黄瓜和水稻种子进行的植物毒性测试表明，与蒸馏水相比，所产生的中间体没有显著毒性。本研究阐明了联合体ZZ对偶氮染料降解的关键贡献，并为嗜盐嗜热条件下的偶氮染料处理提供了新的理论见解和实践指导。
