Biofuel and Renewable Energy Research Center, Department of Biotechnology, Faculty of Chemical Engineering, Babol Noshirvani University of Technology, Babol, Iran.
Biofuel and Renewable Energy Research Center, Department of Biotechnology, Faculty of Chemical Engineering, Babol Noshirvani University of Technology, Babol, Iran.
Environ Res. 2023 Nov 1;236(Pt 2):116801. doi: 10.1016/j.envres.2023.116801. Epub 2023 Aug 8.
A novel and first electrochemical biosensor based on Deoxyribonucleic acid (DNA) as a biological component to measure an antimigraine drug, rizatriptan benzoate (RZB) for patients under treatment in biological samples was developed. A carbon paste electrode (CPE) was modified by calf thymus (CT) double-stranded (ds)-DNA, nickel ferrite magnetic nanoparticles (NiFeONPs), and gold nanoparticles (AuNPs). The morphology of the CT-DNA/NiFeONPs/AuNPs/CPE was characterized by Field emission scanning electron microscope (FESEM). The presence of NiFeONPs and AuNPs was confirmed by energy-dispersive X-ray spectroscopy (EDS) image of the NiFeONPs/AuNPs/CPE surface. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were used to determine the structure and electrochemical characteristics of the CT-DNA/NiFeONPs/AuNPs/CPE. Differential pulse voltammetry (DPV) was used to investigate the electrochemical behavior of RZB. Chronoamperometry (CA) was applied to study the effect of CT-DNA immobilization time on the peak oxidation current of RZB accumulated on the surface of the CT-DNA/NiFeONPs/AuNPs/CPE. The results showed that, under optimum conditions, the prepared electrode responded linearly to RZB concentrations between 0.01 and 2.0 μM, with a 0.0033 μM detection limit (LOD) and 0.01 μM limit of quantification (LOQ). The parameters influencing the biosensor performance (temperature, CT-DNA immobilization time, and RZB/CT-DNA accumulation time) were optimized. DPV showed the displacement of the peak potential towards positive values and the reduction of its current, indicating that the drug could intercalate between the guanine base pairs of CT-DNA. Our biosensor was successfully applied for RZB measurement in human urine, blood serum, plasma samples, and tablets. The presented biosensor was fast response, sensitive, selective, cost-effective, and easy-to-use for RZB determination in pharmaceutical formulations and biological samples.
基于脱氧核糖核酸 (DNA) 作为生物成分，开发了一种新颖的、首创的电化学生物传感器，用于测量生物样品中接受治疗的偏头痛药物苯扎曲普坦苯甲酸酯 (RZB)。通过小牛胸腺 (CT) 双链 (ds)-DNA、镍铁氧体磁性纳米粒子 (NiFeONPs) 和金纳米粒子 (AuNPs) 对碳糊电极 (CPE) 进行修饰。通过场发射扫描电子显微镜 (FESEM) 对 CT-DNA/NiFeONPs/AuNPs/CPE 的形态进行了表征。通过 NiFeONPs/AuNPs/CPE 表面的能量色散 X 射线光谱 (EDS) 图像证实了 NiFeONPs 和 AuNPs 的存在。电化学阻抗谱 (EIS) 和循环伏安法 (CV) 用于确定 CT-DNA/NiFeONPs/AuNPs/CPE 的结构和电化学特性。差分脉冲伏安法 (DPV) 用于研究 RZB 的电化学行为。计时安培法 (CA) 用于研究 CT-DNA 固定时间对 RZB 在 CT-DNA/NiFeONPs/AuNPs/CPE 表面积累的峰氧化电流的影响。结果表明，在最佳条件下，制备的电极对 0.01 至 2.0 μM 范围内的 RZB 浓度呈线性响应，检测限 (LOD) 为 0.0033 μM，定量限 (LOQ) 为 0.01 μM。优化了影响生物传感器性能的参数（温度、CT-DNA 固定时间和 RZB/CT-DNA 积累时间）。DPV 显示峰电位向正值移动，电流减小，表明药物可以嵌入 CT-DNA 的鸟嘌呤碱基对之间。我们的生物传感器成功地用于测定人尿、血清和血浆样品以及片剂中的 RZB。所提出的生物传感器具有快速响应、灵敏、选择性、经济高效且易于使用的特点，可用于药物制剂和生物样品中 RZB 的测定。
