Institute of Nano Science and Nano Technology, University of Kashan, Kashan, P.O. Box 87317-51167, Iran.
Institute of Nano Science and Nano Technology, University of Kashan, Kashan, P.O. Box 87317-51167, Iran; Department of Analytical Chemistry, Faculty of Chemistry, University of Kashan, Kashan, Iran.
Chemosphere. 2023 Oct;338:139427. doi: 10.1016/j.chemosphere.2023.139427. Epub 2023 Jul 5.
Electrochemical methods have low toxicity, fast response and, easy operation. By modifying electrochemical sensors with a conductive and porous modifier, their sensitivity and selectivity can be improved. Nanomaterials with new and extraordinary properties are a new approach in science and especially in electrochemical sensors. In this study, UiO66-NH/mesoporous carbon nitride (M - CN) composite provides a porous structure for decorated Au nanoparticles (AuNPs) to prepare a potent modifier for carbon paste electrode (CPE). Due to environmental toxicity of methotrexate, its sensitive, fast and, low-cost determination in workplace environments is of great interest. So, the modified CPE was applied as a sensitivity analysis approach for methotrexate in plasma samples. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were used as techniques to optimize the analysis and measurement of methotrexate. To measure this drug, several effective parameters were optimized and a calibration curve was drawn under optimal conditions. The calibration curve showed a linear range from 0.5 to 150 μM with a detection limit of 0.15 μM for methotrexate. Examining the repeatability of the response of one electrode and multiple electrodes under optimal conditions shows the high precision of the developed method. Finally, this developed method based on UiO66-NH/M-gCN/AuNPs|CPE was used to determine the methotrexate in the plasma sample using the standard addition method.
电化学方法具有低毒性、快速响应和易于操作的特点。通过用导电和多孔修饰剂修饰电化学传感器，可以提高其灵敏度和选择性。具有新颖和非凡性质的纳米材料是科学，尤其是电化学传感器的新方法。在这项研究中，UiO66-NH/介孔氮化碳（M-CN）复合材料为修饰的金纳米粒子（AuNPs）提供了多孔结构，以制备碳糊电极（CPE）的有效修饰剂。由于甲氨蝶呤的环境毒性，因此在工作环境中对其进行灵敏、快速且低成本的测定具有重要意义。因此，修饰后的 CPE 被用作分析和测量血浆样品中甲氨蝶呤的灵敏度分析方法。循环伏安法（CV）和差分脉冲伏安法（DPV）被用作优化分析和测量甲氨蝶呤的技术。为了测量这种药物，优化了几个有效参数，并在最佳条件下绘制了校准曲线。校准曲线显示，甲氨蝶呤的线性范围为 0.5 至 150 μM，检测限为 0.15 μM。在最佳条件下检查一个电极和多个电极的响应重复性表明该方法具有较高的精度。最后，基于 UiO66-NH/M-gCN/AuNPs|CPE 的这种开发方法被用于使用标准添加法测定血浆样品中的甲氨蝶呤。
