Plant Physiology and Biochemistry Section, Department of Botany, Aligarh Muslim University, Aligarh, 202002, India.
Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia.
Sci Rep. 2023 Aug 9;13(1):12924. doi: 10.1038/s41598-023-40210-9.
Peppermint has gained a promising status due to the presence of a high proportion of bioactive compounds, especially menthol. Due to its pharmacological efficacy, the demand for its plant-based bioactive compounds necessitates its cultivation worldwide. Brassinosteroids are polyhydroxylated sterol derivatives that regulate diverse processes and control many agronomic traits during plant growth and development. A factorial randomised pot experiment was performed in the net house to investigate the effect of 24-Epibrassinolide (EBL) on the growth, physiology, essential oil content, stomatal behaviour and trichome development of the three cultivars of peppermint. Four levels of foliage-applied EBL, viz. 0, 10, 10 and 10 M were applied to the three cultivars of peppermint (Kukrail, Pranjal and Tushar). Among the different treatments of EBL, the application of 10 M increased shoot length by 38.84, 37.59 and 36.91%, root length by 36.73, 29.44 and 33.47%, chlorophyll content by 24.20, 22.48 and 23.32%, P by 32.88, 32.61 and 33.61%, EO content by 32.72, 30.00 and 28.84%, EO yield per plant by 66.66, 77.77 and 73.33% and menthol yield per plant by 127.27, 110 and 118.18% in Kukrail, Tushar and Pranjal respectively, compared with their respective control plants. Further, the 10 M EBL exhibited improved trichome size and density, cellular viability and menthol content of the oil analysed from scanning electron microscopy, confocal laser scanning microscopy and GC-MS respectively as compared to the control. In conclusion, out of different levels of EBL, two sprays of 10 M EBL proved effective in enhancing the morphophysiological features and productivity of mint plants, particularly for cultivar Kukrail.
薄荷因其含有高比例的生物活性化合物而备受关注，特别是薄荷醇。由于其药理学功效，对其植物源生物活性化合物的需求促使其在全球范围内种植。油菜素内酯是一种多羟基甾醇衍生物，可调节植物生长发育过程中的多种过程，并控制许多农艺性状。在网室内进行了一项因子随机盆栽试验，以研究 24-表油菜素内酯（EBL）对三种薄荷品种生长、生理、精油含量、气孔行为和毛状体发育的影响。将四个叶面喷施 EBL 水平（0、10、10 和 10 M）应用于三种薄荷品种（Kukrail、Pranjal 和 Tushar）。在不同的 EBL 处理中，应用 10 M 可使茎长分别增加 38.84%、37.59%和 36.91%，根长分别增加 36.73%、29.44%和 33.47%，叶绿素含量分别增加 24.20%、22.48%和 23.32%，P 分别增加 32.88%、32.61%和 33.61%，精油含量分别增加 32.72%、30.00%和 28.84%，每株植物的精油产量分别增加 66.66%、77.77%和 73.33%，每株植物的薄荷醇产量分别增加 127.27%、110%和 118.18%，与对照植物相比。此外，与对照相比，10 M EBL 处理表现出改善的毛状体大小和密度、细胞活力和油中的薄荷醇含量，这分别通过扫描电子显微镜、共聚焦激光扫描显微镜和 GC-MS 分析得出。总之，在不同的 EBL 水平中，两次喷施 10 M EBL 被证明可有效增强薄荷植物的形态生理特征和生产力，特别是对 Kukrail 品种。
