Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA.
Department of Ophthalmology, The First Affiliated Hospital of Jinan University, Guangzhou, China.
Methods Mol Biol. 2023;2708:115-121. doi: 10.1007/978-1-0716-3409-7_12.
Real-time imaging of retinal ganglion cells (RGCs) provides an opportunity for detailed investigation of retinal development, disease mechanisms, and the evaluation of interventions affecting ocular structures. Here we use a transgenic model to describe a step-by-step protocol for visualizing RGC survival in real-time by using confocal scanning laser ophthalmoscopy (cSLO).
实时成像视网膜神经节细胞(RGCs)为详细研究视网膜发育、疾病机制以及评估影响眼结构的干预措施提供了机会。在这里,我们使用转基因模型描述了一个通过共焦扫描激光检眼镜(cSLO)实时可视化 RGC 存活的逐步协议。