通过共焦扫描激光眼科显微镜对视网膜细胞凋亡进行实时成像。

Real-time imaging of retinal cell apoptosis by confocal scanning laser ophthalmoscopy.

作者信息

Normando Eduardo M, Dehabadi Mohammad H, Guo Li, Turner Lisa A, Pollorsi Gaia, Cordeiro M Francesca

机构信息

Glaucoma & Retinal Degeneration Research Group, Visual Neurosciences, UCL Institute of Ophthalmology, 11-43 Bath Street, London, EC1V 9EL, UK.

出版信息

Methods Mol Biol. 2015;1254:227-37. doi: 10.1007/978-1-4939-2152-2_17.

DOI:10.1007/978-1-4939-2152-2_17

PMID:25431069

Abstract

Retinal cell apoptosis occurs in many eye conditions, including glaucoma, diabetic retinopathy and Alzheimer's disease. Real-time detection of retinal cell apoptosis has potential clinical value in early disease detection, as well as evaluating disease progression and treatment efficacy. Here, we describe our novel imaging technology DARC (Detection of Apoptosing Retinal Cells), which can be used to visualize single retinal neurons undergoing apoptosis in real time, by using fluorescently labeled Annexin A5 and confocal scanning laser ophthalmoscopy (cSLO ). Clinical trials of DARC in glaucoma patients are due to start shortly, but in this chapter, we describe this technique in experimental animal models.

摘要

视网膜细胞凋亡发生于多种眼部疾病中，包括青光眼、糖尿病性视网膜病变和阿尔茨海默病。视网膜细胞凋亡的实时检测在疾病早期检测、评估疾病进展及治疗效果方面具有潜在临床价值。在此，我们介绍我们的新型成像技术DARC（凋亡视网膜细胞检测），该技术可通过使用荧光标记的膜联蛋白A5和共焦扫描激光检眼镜（cSLO）实时可视化单个正在经历凋亡的视网膜神经元。DARC在青光眼患者中的临床试验即将开始，但在本章中，我们在实验动物模型中描述该技术。