But Valeriu Mihai, Bulboacă Adriana Elena, Rus Vasile, Ilyés Tamás, Gherman Mădălina Luciana, Bolboacă Sorana D
Department of Medical Informatics and Biostatistics, "Iuliu Haţieganu" University of Medicine and Pharmacy, Louis Pasteur Street, No. 6, Cluj-Napoca, 400349, Romania.
Department of Pathophysiology, "Iuliu Haţieganu" University of Medicine and Pharmacy, Victor Babeş Street, No. 2-4, Cluj-Napoca, 400012, Romania.
Thromb J. 2023 Aug 9;21(1):85. doi: 10.1186/s12959-023-00516-0.
Lavender oil (LO) possesses anti-inflammatory, antioxidant, antifungal, antibacterial, sedative, cardio-protective, and antinociceptive properties. Thrombosis and inflammation are interplayed processes that interact and influence one another. Our research compared three routes of administration to assess the efficacy of pretreatment with LO on carrageenan-induced thrombosis in rat tail.
Wistar-Bratislava white rats were randomly divided into five groups of ten rats each and pretreated 3 consecutive days prior the inducement of thrombosis to with one dose of LO (150 mg/kg body weight (b.w.)): per os by gavage (TLOPO group), intraperitoneal (TIPLO group) and subcutaneous (TSCLO group). We also have a control (C, received saline solution 0.9% and DMSO (vehicle) 1 ml intraperitoneal (i.p.)) group and a group with thrombosis (T group, received saline solution 0.9% plus vehicle 1 ml i.p.). Histopathological examinations were conducted together with measurements of the circulating levels of three oxidative stress markers, antioxidant effect (TAC and THIOL), and three proinflammatory cytokines (TNF- α, RANTES, and MCP-1).
When administered intraperitoneally, lavender oil has the best efficacy on circulating levels of oxidative stress parameters (MDA, NOx, TOS), one oxidative stress marker (THIOL), and all studied proinflammatory cytokines (p-values < 0.02). Moreover, TIPLO displayed the closest values for bleeding and clotting time to the C group, as well as the lowest length of the thrombus than the T, TPOLO, and TSCLO groups (p-values < 0.001). The TIPLO group has histological appearance comparable to the C group, with the exception of the presence of oedema.
Lavender oil pretreatment with intraperitoneal administration as three days, one-dose per day, showed anti-inflammatory and antioxidant efficacy in experimentally induced thrombosis.
薰衣草油（LO）具有抗炎、抗氧化、抗真菌、抗菌、镇静、心脏保护和抗伤害感受特性。血栓形成和炎症是相互作用、相互影响的过程。我们的研究比较了三种给药途径，以评估LO预处理对大鼠尾部角叉菜胶诱导的血栓形成的疗效。
将Wistar-布拉迪斯拉发白色大鼠随机分为五组，每组十只，在诱导血栓形成前连续三天用一剂LO（150mg/kg体重（b.w.））进行预处理：经口灌胃（TLOPO组）、腹腔内注射（TIPLO组）和皮下注射（TSCLO组）。我们还有一个对照组（C组，腹腔内注射（i.p.）0.9%生理盐水和1ml二甲基亚砜（溶媒））和一个血栓形成组（T组，腹腔内注射（i.p.）0.9%生理盐水加1ml溶媒）。进行了组织病理学检查，并测量了三种氧化应激标志物的循环水平、抗氧化作用（TAC和硫醇）以及三种促炎细胞因子（TNF-α、RANTES和MCP-1）。
腹腔内给药时，薰衣草油对氧化应激参数（MDA、NOx、TOS）、一种氧化应激标志物（硫醇）和所有研究的促炎细胞因子的循环水平具有最佳疗效（p值<0.02）。此外，TIPLO组的出血和凝血时间值与C组最接近，并且与T组、TPOLO组和TSCLO组相比，血栓长度最短（p值<0.001）。TIPLO组的组织学外观与C组相当，但存在水肿。
每天一剂，连续三天腹腔内给予薰衣草油预处理，在实验诱导的血栓形成中显示出抗炎和抗氧化功效。
