Fleischhacker M, Böhme H
Z Allg Mikrobiol. 1979;19(1):3-8. doi: 10.1002/jobm.3630190102.
The task of this work was the establishment of an effective transfer system for F'-plasmids from Escherichia coli to Proteus mirabilis. It is shown that cells of PG VI act as recipients in crosses with E. coli F' strains but with a low transfer rate of the plasmid. The presumption that a restriction -- modification system in P. mirabilis was the only reason for the low transfer could not be confirmed. An indirect selection method was developed to isolate P. mirabilis cells which are better recipients. Conjugation experiments showed that the isolated mutants had a better recipient capacity (increase of about 100). This is true not only for the transfer of a F'-plasmid but also for a R-plasmid. The stability of these plasmids in the mutant cells, however, was much lower than in the wild type.
这项工作的任务是建立一种有效的F'-质粒从大肠杆菌转移至奇异变形杆菌的转移系统。结果表明,PG VI细胞在与大肠杆菌F'菌株杂交时可作为受体,但质粒的转移率较低。奇异变形杆菌中的限制-修饰系统是转移率低的唯一原因这一推测无法得到证实。开发了一种间接选择方法来分离作为更好受体的奇异变形杆菌细胞。接合实验表明,分离出的突变体具有更好的受体能力(提高了约100倍)。这不仅适用于F'-质粒的转移,也适用于R-质粒的转移。然而,这些质粒在突变体细胞中的稳定性远低于野生型细胞。