Berenjian Atefeh, Bakhtiarizadeh Mohammad Reza, Mohammadi-Sangcheshmeh Abdollah, Sharifi Seyed Davood
Department of Animal and Poultry Science, Faculty of Agricultural Technology, College of Agriculture and Natural Resources, University of Tehran, Tehran, Iran.
Front Physiol. 2023 Jul 25;14:1198247. doi: 10.3389/fphys.2023.1198247. eCollection 2023.
Supplement of ω-3 fatty acids can decrease the harmful effects of stress. However, the potential molecular mechanisms that are modulated by dietary ω-3 fatty acids in laying hens under stress remain unknown. Hence, RNA-sequencing (RNA-Seq) technology was used to gain new insights into different gene expression profiles and potential pathways involved in response to stress in the liver of 35-week-old Lohmann LSL-Lite laying hens supplemented with ω-3. Three groups including control (non-stress), stress, and stress_ω-3 fatty acids (three layers per each group) were applied. A total of 1,321 genes were detected as differentially expressed genes of which 701, 1,049, and 86 DEGs belonged to stress vs. control, stress_ω-3 vs. control, and stress vs. stress_ω-3 pairwise comparisons, respectively. Gene ontology and KEGG pathway analysis indicated that the DEGs were enriched in particular regulation of steroid and cholesterol biosynthetic process, fatty acid degradation, AMPK signaling pathway, fatty acid biosynthesis, and immune response. Our data represented a promising approach regarding the importance of ω-3 as anxiolytic and anti-stress. In this context, and genes were downregulated in the stress_ω-3 group compared to the stress group, which are associated with decreased activity of glutamatergic stimulatory neurons and probably play important role in facilitating the response to stress. This study extends the current understanding of the liver transcriptome response to physiological stress, and provides new insights into the molecular responses to stress in laying hens fed a diet supplemented with ω-3 fatty acids.
补充ω-3脂肪酸可以减轻应激的有害影响。然而，日粮ω-3脂肪酸在应激蛋鸡中所调控的潜在分子机制仍不清楚。因此，本研究利用RNA测序（RNA-Seq）技术，对35周龄补充ω-3的罗曼LSL-Lite蛋鸡肝脏中参与应激反应的不同基因表达谱和潜在途径有了新的认识。实验设置了三组，包括对照组（非应激）组、应激组和应激+ω-3脂肪酸组（每组三只蛋鸡）。共检测到1321个差异表达基因，其中分别有701、1049和86个差异表达基因属于应激组与对照组、应激+ω-3脂肪酸组与对照组以及应激组与应激+ω-3脂肪酸组的两两比较。基因本体论和KEGG通路分析表明，差异表达基因富集于类固醇和胆固醇生物合成过程、脂肪酸降解、AMPK信号通路、脂肪酸生物合成以及免疫反应的特定调控。我们的数据表明ω-3具有抗焦虑和抗应激作用，这是一种很有前景的方法。在此背景下，与应激组相比，应激+ω-3脂肪酸组中 和 基因表达下调，这与谷氨酸能兴奋性神经元活性降低有关，可能在促进应激反应中起重要作用。本研究扩展了目前对肝脏转录组对应激生理反应的理解，并为补充ω-3脂肪酸日粮的蛋鸡应激分子反应提供了新的见解。
