Suppr超能文献

对1897年(豆科)普兰(Roxb.)全质体基因组的分析。

Analysis of the complete plastomes of (Roxb.) Prain 1897 (Fabaceae).

作者信息

Yang Heyu, Jiang Mei, Sun Sihui, Liu Shengyu, Chen Haimei, Du Qing, Wang Bin, Li Yong, Wang Liqiang, Liu Chang

机构信息

Key Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine from Ministry of Education, Engineering Research Center of Chinese Medicine Resources from Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, P. R. China.

College of Pharmacy, Xiangnan University, Chenzhou, P. R. China.

出版信息

Mitochondrial DNA B Resour. 2023 Aug 7;8(8):841-846. doi: 10.1080/23802359.2023.2241684. eCollection 2023.

Abstract

(Roxb.) Prain 1897, belonging to the family Fabaceae, is not only a landscape tree but also a medicinal plant. At present, few plastomes have been reported from , which delays the in-depth phylogenomic studies and the development of high-resolution discriminating markers for this genus. Herein, we sequenced the first plastome of by NGS technology. The genome is a circular structure (176,158 bp), containing a large single-copy (LSC) region (91,521 bp), a small copy (SSC) region (5237 bp), and two inverted repeat (IR) regions (39,700 bp each). It has 35.45% GC content and encodes 109 unique genes, which are 76 protein-coding, 4 rRNA, and 29 tRNA genes. The genetic distance analysis of the intergenic spacer regions for , and shows four intergenic regions with very high K2p values, namely, A-D (15.04), tK-16 (10.77), 11-36 (17.63) and 3-19 (20.08), which can discriminate the three species. In addition, we identified ten pairs of regions that could be utilized to design primers to discriminate the three species. The phylogenetic analysis showed was closely related to . The results in this study will provide valuable information to elucidate the classification, identification and evolutionary history of .

摘要

(豆科罗克斯伯里属)普拉因1897年,不仅是一种景观树,也是一种药用植物。目前,该属报道的质体基因组很少,这延迟了其深入的系统基因组学研究以及该属高分辨率鉴别标记的开发。在此,我们通过二代测序技术对该属的首个质体基因组进行了测序。该基因组为环状结构(176,158 bp),包含一个大单拷贝(LSC)区域(91,521 bp)、一个小单拷贝(SSC)区域(5,237 bp)和两个反向重复(IR)区域(各39,700 bp)。其GC含量为35.45%,编码109个独特基因,其中包括76个蛋白质编码基因、4个rRNA基因和29个tRNA基因。对该属、及的基因间隔区进行遗传距离分析,结果显示有四个基因间隔区具有非常高的K2p值,即A - D(15.04)、tK - 16(10.77)、11 - 36(17.63)和3 - 19(20.08),这些区域可区分这三个物种。此外,我们鉴定出十对可用于设计引物以区分这三个物种的区域。系统发育分析表明与密切相关。本研究结果将为阐明该属的分类、鉴定和进化历史提供有价值的信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a34e/10408564/697733613dd0/TMDN_A_2241684_F0001_C.jpg

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验