Li Jingling, Jiang Mei, Wang Liqiang, Yu Jie, Chen Haimei, Liu Jingting, Liu Chang
College of Horticulture and Landscape Architecture, Southwest University, Chongqing, China.
Key Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine from Ministry of Education, Engineering Research Center of Chinese Medicine Resources from Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.
Mitochondrial DNA B Resour. 2021 Apr 19;6(4):1431-1439. doi: 10.1080/23802359.2021.1911698.
is a common ornamental arbor in the genus (family Rosaceae). Here, we sequenced and assembled the complete plastome of using the next-generation DNA sequencing technology. And we then compared it with nine species using a range of bioinformatics software tools. The ten plastomes had sizes ranging from 159,230 bp for to 160,346 bp for . They all had a conservative quartile structure. It contained two single-copy regions: a large single-copy (LSC) region, a small single-copy (SSC) region, and a pair of inverted repeat (IR) regions. Each of the plastomes encoded 113 unique genes, including 79 protein-coding genes, four rRNA genes, and 30 tRNA genes. Furthermore, we detected six hypervariable regions (K-16, B-C, T-D, C-V, E-L, F-32-L), which could be used as potential molecular markers. We constructed two phylogenetic trees with plastomes or concatenated protein sequences of 25 species of 8 genera of Rosaceae. The tree constructed with complete plastomes has much stronger support. The results placed in the upper part of the phylogenetic tree. It shows that and are closely related. In summary, the plastomes of are conserved overall but carry significant minor variations, as expected. The results will be indispensable for distinguishing species, understanding the interspecific diversity, and elucidating the evolutionary processes of species.
是蔷薇科（Rosaceae）属一种常见的观赏乔木。在此，我们使用下一代DNA测序技术对其完整叶绿体基因组进行了测序和组装。然后，我们使用一系列生物信息学软件工具将其与九个物种进行了比较。这十个叶绿体基因组的大小从[物种名称1]的159,230 bp到[物种名称2]的160,346 bp不等。它们都具有保守的四分体结构。它包含两个单拷贝区域：一个大单拷贝（LSC）区域、一个小单拷贝（SSC）区域和一对反向重复（IR）区域。每个叶绿体基因组编码113个独特基因，包括79个蛋白质编码基因、4个rRNA基因和30个tRNA基因。此外，我们检测到六个高变区域（K - 16、B - C、T - D、C - V、E - L、F - 32 - L），它们可作为潜在的分子标记。我们用蔷薇科8属25种植物的叶绿体基因组或串联蛋白质序列构建了两棵系统发育树。用完整叶绿体基因组构建的树支持度更强。结果将[物种名称]置于系统发育树的上部。这表明[物种名称]与[另一物种名称]密切相关。总之，[物种名称]的叶绿体基因组总体上是保守的，但存在明显的微小变异，正如预期的那样。这些结果对于区分物种、理解种间多样性以及阐明[物种名称]的进化过程将是不可或缺的。
