维生素D结合蛋白（GC）和维生素D受体（VDR）基因多态性对先兆子痫发生风险的影响。
Impact of vitamin D binding protein (GC) and vitamin D receptor (VDR) gene polymorphism on the risk of developing preeclampsia.
作者信息
Rahman Md Mostafijur, Nawfal Tamima, Khabir Fabliha Afiea, Hosen Md Bayejid, Washif Mubasshir, Kabir Yearul, Howlader M Zakir Hossain
机构信息
Laboratory of Nutrition and Health Research, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, 1000, Bangladesh.
Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, 1000, Bangladesh.
出版信息
Biochem Biophys Rep. 2023 Aug 4;35:101526. doi: 10.1016/j.bbrep.2023.101526. eCollection 2023 Sep.
OBJECTIVE
Preeclampsia is a multifactorial disease characterized by high blood pressure and protein in the urine. In this study, we investigated the association of vitamin D binding protein (GC) and vitamin D receptor (VDR) gene polymorphism with the risk of developing preeclampsia.
METHODS
25-hydroxyvitamin D was measured using High-performance Liquid Chromatography. Vitamin D binding protein and vitamin D receptor gene polymorphisms were determined by polymerase chain reaction-restriction fragment length polymorphism.
RESULTS
The control subjects have significant higher level of 25-hydroxyvitamin D (33.5 ± 1.194 ng/mL) relative to patients (23.97 ± 1.604 ng/mL) (p < 0.05). Vitamin D receptor rs1544410 and rs2228570 dominant model (GA + AA; TC + CC) showed significant higher risk of developing Preeclampsia (OR = 4.11, 95% CI = 0.62-27.09, p < 0.01; OR = 3.58, 95%CI = 0.78-16.38, p < 0.001 respectively). Similarly, vitamin D binding protein rs7041 and rs4588, dominant model (TG + GG; CA + AA) showed higher risk of preeclampsia development compared to control people (OR = 1.69, 95%CI = 0.35-8.19, p < 0.05; OR = 1.06, 95%CI = 0.25-4.44, p < 0.05 respectively). AA genotype of rs4588 of GC gene was significantly associated with 25-hydroxyvitamin D level in serum relative to CC and CA (p < 0.05).
CONCLUSION
From our study, we can conclude that a low level of 25-hydroxyvitamin D, GC (rs1544410 and rs2228570), and VDR (rs4588 and rs7041) gene polymorphism is linked with an increased risk of developing preeclampsia.
目的
子痫前期是一种以高血压和蛋白尿为特征的多因素疾病。在本研究中，我们调查了维生素D结合蛋白（GC）和维生素D受体（VDR）基因多态性与子痫前期发病风险的关联。
方法
采用高效液相色谱法测定25-羟基维生素D。通过聚合酶链反应-限制性片段长度多态性确定维生素D结合蛋白和维生素D受体基因多态性。
结果
与患者（23.97±1.604 ng/mL）相比，对照组受试者的25-羟基维生素D水平显著更高（33.5±1.194 ng/mL）（p<0.05）。维生素D受体rs1544410和rs2228570显性模型（GA+AA；TC+CC）显示子痫前期发病风险显著更高（OR=4.11，95%CI=0.62-27.09，p<0.01；OR=3.58，95%CI=0.78-16.38，p<0.001）。同样，维生素D结合蛋白rs7041和rs4588显性模型（TG+GG；CA+AA）与对照组相比，子痫前期发病风险更高（OR=1.69，95%CI=0.35-8.19，p<0.05；OR=1.06，95%CI=0.25-4.44，p<0.05）。相对于CC和CA，GC基因rs4588的AA基因型与血清25-羟基维生素D水平显著相关（p<0.05）。
结论
从我们的研究中可以得出结论，低水平的25-羟基维生素D、GC（rs1544410和rs2228570）以及VDR（rs4588和rs7041）基因多态性与子痫前期发病风险增加有关。
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