Long Mengfei, Pei Xiaomei, Lu Zhi, Xu Duo, Zheng Nan, Li Yaxian, Ge Hanxiao, Cao Wentao, Osire Tolbert, Xia Xiaole
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Infinitus (China) Co. Ltd., Guangzhou, 510665, China.
Heliyon. 2023 Jul 26;9(8):e18735. doi: 10.1016/j.heliyon.2023.e18735. eCollection 2023 Aug.
are widely used around the world, mainly in purging and removal of endogenous active substances, such as anthraquinone and its derivatives. However, the potential toxicity of anthraquinones to the liver, kidney, and intestinal limits the application of . In this study, we aimed at safe regulation of to degrade anthraquinones, boosting medicinal properties and reducing toxicity and potency with fermentation strains H1102 for fermentation were selected as the initial strain which was capable of producing high yields of functional pigment and low yields of hazardous citrinin. The anthraquinone degradation rate reached 41.2%, with 212.2 U mL of the pigment and approximately 0.038 mg L of the citrinin under optimal fermentation conditions followed by response surface streamlining, which met the requirements of reducing toxicity, increasing efficiency of fermented . Furthermore, the culture had no observable toxic effect on HK-2 and L-02 cells and further inhibited cell apoptosis and necrosis. Overall, our results showed that fermentation could provide an alternative strategy for toxicity reduction of herbal medicines as well as efficacy enhancement.
在世界各地被广泛使用，主要用于清除和去除内源性活性物质，如蒽醌及其衍生物。然而，蒽醌对肝脏、肾脏和肠道的潜在毒性限制了其应用。在本研究中，我们旨在通过安全调控以降解蒽醌，增强药用特性并降低毒性和效力，选用发酵菌株H1102作为发酵的初始菌株，该菌株能够高产功能性色素且低产有害桔霉素。在最佳发酵条件下，蒽醌降解率达到41.2%，色素产量为212.2 U/mL，桔霉素产量约为0.038 mg/L，随后通过响应面优化，满足了降低毒性、提高发酵效率的要求。此外，发酵培养物对HK-2和L-02细胞没有明显的毒性作用，进一步抑制了细胞凋亡和坏死。总体而言，我们的结果表明发酵可为降低草药毒性以及提高疗效提供一种替代策略。
