Peng Lin, Ai-Lati Aisikaer, Liu Shuangping, Ji Zhongwei, Mao Jian, Che Xin
National Engineering Laboratory for Cereal Fermentation Technology Jiangnan University Wuxi China.
School of Food Science and Technology Jiangnan University Wuxi China.
Food Sci Nutr. 2020 Mar 17;8(4):2134-2142. doi: 10.1002/fsn3.1511. eCollection 2020 Apr.
Monacolin K (MK) is a secondary metabolite synthesized by polyketide synthases of spp. In this study, the combined supplementation of three medicines, including Citri Reticulatae Pericarpium (CRP), Fructus crataegi (FC), and (RAD), were mixed with nonglutinous rice and were optimized by response surface methodology to enhance the production of MK in fermented red mold rice (RMR). Under the optimum condition, MK production achieved 3.60 mg/g, which was 41.18% higher than RMR without medicines. The improved MK production was mainly caused by the up-regulated transcription level of , , , , , and . Meanwhile, the inhibitory effect of (PC) on MK production (only 0.436 mg/g) was caused by significantly down-regulated transcription of six tested genes. Therefore, this study is beneficial for better understanding of the possible mechanism of enhanced MK production by optimization of fermentation conditions.
莫纳可林K（MK）是由[具体菌种]的聚酮合酶合成的次生代谢产物。在本研究中，将包括陈皮（CRP）、山楂（FC）和[具体药材名称]（RAD）在内的三种药物与糯米混合，并通过响应面法进行优化，以提高发酵红米（RMR）中MK的产量。在最佳条件下，MK产量达到3.60mg/g，比未添加药物的RMR高出41.18%。MK产量的提高主要是由于[相关基因名称1]、[相关基因名称2]、[相关基因名称3]、[相关基因名称4]、[相关基因名称5]和[相关基因名称6]转录水平的上调。同时，[具体物质名称]（PC）对MK产量的抑制作用（仅0.436mg/g）是由六个测试基因的转录显著下调引起的。因此，本研究有助于更好地理解通过优化发酵条件提高MK产量的可能机制。
