Clin Lab. 2023 Aug 1;69(8). doi: 10.7754/Clin.Lab.2023.220906.
Regenerating gene (REG) family proteins play a pivotal role in cell proliferation, tissue regeneration, and tumor metastasis. Recent studies have concentrated on the role of REG proteins in pancreatic cancer, but the results remain controversial. In this study, a meta-analysis was performed to evaluate the precise diagnostic value of REG proteins in pancreatic cancer.
A search was conducted in PubMed, Medline, Embase, Cochrane Library, Chinese National Knowledge Infrastructure (CNKI), Biomedical Literature Database (CBM), and WANFANG Data up to May 5, 2021. The QUADAS-2 tool was used to evaluate the quality of the included studies. The statistical analysis of the diagnostic tests was conducted using RevMan5 and Meta-Disc 1.4. The pooled sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR), and their 95% confidence intervals (95% CIs) were calculated from each eligible study.
The meta-analysis included 15 articles containing 796 patients and 584 controls. The pooled sensitivity was 0.71 (95% CI: 0.67 - 0.74), the pooled specificity was 0.73 (95% CI: 0.70 - 0.76), and the pooled DOR was 11.35 (95% CI: 5.92 - 21.77), respectively. The overall area under the receiver operating characteristic curve (AUC) was 0.84. Spearman's correlation coefficient was 0.34 (p = 0.221). For the subgroup analysis, the REG4 protein showed higher diagnostic accuracy compared with the other REG proteins.
REG proteins have moderate diagnostic accuracy in pancreatic cancer. Further well-designed studies with larger sample sizes and clinical application are needed to validate the results of this meta-analysis.
再生基因（REG）家族蛋白在细胞增殖、组织再生和肿瘤转移中发挥关键作用。最近的研究集中在 REG 蛋白在胰腺癌中的作用，但结果仍存在争议。本研究通过荟萃分析评估 REG 蛋白在胰腺癌中的精确诊断价值。
检索 PubMed、Medline、Embase、Cochrane 图书馆、中国知网（CNKI）、中国生物医学文献数据库（CBM）和万方数据，检索时间截至 2021 年 5 月 5 日。采用 QUADAS-2 工具评价纳入研究的质量。使用 RevMan5 和 Meta-Disc 1.4 对诊断试验的统计分析进行汇总。从每项合格研究中计算合并敏感性、特异性、阳性似然比（PLR）、阴性似然比（NLR）、诊断比值比（DOR）及其 95%置信区间（95%CI）。
荟萃分析纳入了 15 项包含 796 例患者和 584 例对照的研究。合并敏感性为 0.71（95%CI：0.67-0.74），合并特异性为 0.73（95%CI：0.70-0.76），合并 DOR 为 11.35（95%CI：5.92-21.77）。受试者工作特征曲线下总面积（AUC）为 0.84。Spearman 相关系数为 0.34（p=0.221）。亚组分析显示，REG4 蛋白的诊断准确性高于其他 REG 蛋白。
REG 蛋白在胰腺癌中具有中等诊断准确性。需要进一步开展设计良好、样本量大且具有临床应用价值的研究来验证本荟萃分析的结果。
