Clin Lab. 2023 Aug 1;69(8). doi: 10.7754/Clin.Lab.2023.230214.
Blood count reference intervals are important to diagnose diseases and assess overall health, especially for young children. Although, in 2021, the National Health Commission of the People's Republic of China issued "Reference intervals of blood cell analysis for children (WS/T 779-2021)", these RIs may not suitable for small children all over the country due to racial, lifestyle, and geographical differences. The aim of this study was to establish and validate locally determined hematological reference intervals among young children in Nantong district and compare them with WS/T 779-2021 and American data.
The reference sample consisted of 4,758 apparently healthy small children aged from age 28 days to 3 years according to the EP28-A3c guideline issued by the Clinical and Laboratory Standards Institute (CLSI). Capillary blood samples collected in K2-EDTA anticoagulant tubes analyzed by standard procedures. Statistical analysis was based on the guidelines of the CLSI.
Pediatric reference intervals for 18 capillary complete blood count (CCBC) parameters were established for young children. WBC and differentials did not differ by gender in the combined analysis of all data, but showed some variations among different age groups, especially for NE and LYM. RIs of RBC value, MCV, and MCH were established, especially with regard to the difference among different age and gender groups. An overall increasing trend of PLT value was observed in children with no obvious difference between boys and girls. Further validation with 1,136 healthy subjects demonstrated that the verified proportions of our study were within 90.11% - 100%. RIs determined in the present study were more concentrated than WS/T 779-2021, with slight differences in the upper and bottom boundaries.
Establishing appropriate region-specific reference intervals for pediatrics is essential. This study offers local reference intervals of CCBC values for young children and could be used as a benchmark for similar populations in the Yangtze River Delta economic region.
血球计数参考区间对于诊断疾病和评估整体健康状况非常重要，尤其是对于幼儿。尽管 2021 年，中国国家卫生健康委员会发布了《儿童血细胞分析参考区间》（WS/T 779-2021），但由于种族、生活方式和地理位置的差异，这些参考区间可能并不适用于全国所有的幼儿。本研究旨在建立并验证南通地区幼儿的局部确定的血液学参考区间，并与 WS/T 779-2021 和美国数据进行比较。
参考样本由根据临床和实验室标准协会（CLSI）发布的 EP28-A3c 指南，年龄在 28 天至 3 岁的 4758 名看似健康的幼儿组成。用标准程序采集 K2-EDTA 抗凝管中的毛细血管血样进行分析。统计分析基于 CLSI 的指南。
为幼儿建立了 18 项毛细血管全血细胞计数（CCBC）参数的儿科参考区间。在所有数据的综合分析中，WBC 和不同分类在性别之间没有差异，但在不同年龄组之间存在一些变化，特别是对于 NE 和 LYM。还建立了 RBC 值、MCV 和 MCH 的 RI，特别是考虑到不同年龄和性别组之间的差异。观察到血小板值呈整体上升趋势，男孩和女孩之间没有明显差异。用 1136 名健康受试者进一步验证，表明本研究的验证比例在 90.11%-100%之间。本研究确定的 RI 比 WS/T 779-2021 更为集中，上下边界略有不同。
为儿科制定适当的特定地区参考区间至关重要。本研究提供了南通地区幼儿 CCBC 值的局部参考区间，可作为长三角经济区类似人群的基准。
