Department of Applied Chemistry, University of South Bohemia, Faculty of Agriculture and Technology, České Budějovice, Czech Republic.
Department of Biology, University of South Bohemia, Faculty of Education, České Budějovice, Czech Republic.
Int J Med Mushrooms. 2023;25(8):43-53. doi: 10.1615/IntJMedMushrooms.2023049038.
Pleurotus ostreatus was cultivated on a commercially available wheat straw substrate enriched with Zn and Se. Various amounts of Zn (10, 50, and 100 mg) and Se (1, 5, and 10 mg) in suitable forms ((CH3COO)2Zn·2H2O, Zn(NO3)2·6H2O, and Na2SeO3·5H2O, respectively) were dissolved in 50 ml of deionized water and homogenously nebulized into the substrate block of 2.4 kg weight. The increase in the Zn content in fruiting bodies cultivated on the enriched substrate was relatively low compared with fruiting bodies cultivated on the substrate with no addition at the first flush. The application of different Zn compounds (acetate vs. nitrate) gave similar results. However, the addition of 1 mg of Se into the cultivation substrate block increased the content of Se in fruiting bodies to about 3-6 mg/kg dry matter. This content was one order of magnitude higher compared with the Se content in fruiting bodies harvested from the substrate with no Se addition (< 0.12-0.58 mg/kg dry matter). In the case of the addition of 5 mg of Se, there was a further significant increase in the content of this element to about 40-60 mg/kg dry matter.
糙皮侧耳（Pleurotus ostreatus）在一种商业上可获得的富含锌和硒的小麦秸秆基质上进行培养。将各种量的锌（10、50 和 100mg）和硒（1、5 和 10mg）以合适的形式（（CH3COO）2Zn·2H2O、Zn(NO3)2·6H2O 和 Na2SeO3·5H2O）溶解在 50ml 去离子水中，并均匀地雾化到 2.4kg 重的基质块中。与没有添加的基质相比，在富化基质上培养的子实体中锌含量的增加相对较低。在第一潮时，应用不同的锌化合物（醋酸盐与硝酸盐）得到了相似的结果。然而，向栽培基质块中添加 1mg 的硒可将子实体中的硒含量提高到约 3-6mg/kg 干物质。与未添加硒的基质中收获的子实体中的硒含量（<0.12-0.58mg/kg 干物质）相比，这个含量高了一个数量级。在添加 5mg 硒的情况下，该元素的含量进一步显著增加到约 40-60mg/kg 干物质。
