van Duijn G, Valtersson C, Chojnacki T, Verkleij A J, Dallner G, de Kruijff B
Biochim Biophys Acta. 1986 Oct 9;861(2):211-23. doi: 10.1016/0005-2736(86)90423-2.
The effect of dolichol and dolichyl phosphate on fusion between large unilamellar vesicles comprised of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) was studied using a fluorescence resonance energy transfer assay. The influence of dolichyl phosphate on the transbilayer movement of DOPC in multilamellar vesicles (MLV) and large unilamellar vesicles (LUV) composed of DOPC and DOPE (1:2) was investigated by using the phosphatidylcholine-specific transfer protein. 31P-NMR and freeze-fracture electron microscopy were employed to study the macroscopic organization of DOPC and DOPE containing model membranes in the absence or presence of dolichyl phosphate. The results indicate that both dolichol and dolichyl phosphate enhance vesicle fusion in a comparable and concentration-dependent way; the amount of exchangeable PC from MLVs is increased by dolichyl phosphate, probably as a result of fusion processes; dolichyl phosphate destabilizes the bilayer organization in MLVs comprised of DOPE and DOPC, resulting in the formation of hexagonal (HII) phase and 'lipidic' particles.
使用荧光共振能量转移测定法，研究了多萜醇和磷酸多萜醇对由1,2 - 二油酰 - sn - 甘油 - 3 - 磷酸胆碱（DOPC）和1,2 - 二油酰 - sn - 甘油 - 3 - 磷酸乙醇胺（DOPE）组成的大单层囊泡之间融合的影响。通过使用磷脂酰胆碱特异性转移蛋白，研究了磷酸多萜醇对由DOPC和DOPE（1:2）组成的多层囊泡（MLV）和大单层囊泡（LUV）中DOPC跨膜运动的影响。采用31P - NMR和冷冻断裂电子显微镜来研究在不存在或存在磷酸多萜醇的情况下，含DOPC和DOPE的模型膜的宏观组织。结果表明，多萜醇和磷酸多萜醇均以类似且浓度依赖性的方式增强囊泡融合；磷酸多萜醇增加了来自MLV的可交换PC的量，这可能是融合过程的结果；磷酸多萜醇使由DOPE和DOPC组成的MLV中的双层组织不稳定，导致六方（HII）相和“脂质”颗粒的形成。
