磷酸多萜醇对双层脂质膜通透性和稳定性的影响。

Influence of dolichyl phosphate on permeability and stability of bilayer lipid membranes.

作者信息

Janas T, Tien H T

机构信息

Department of Physiology, Michigan State University, East Lansing 48824.

出版信息

Biochim Biophys Acta. 1988 Apr 22;939(3):624-8. doi: 10.1016/0005-2736(88)90110-1.

DOI:10.1016/0005-2736(88)90110-1

PMID:3355837

Abstract

The ionic permeability coefficients, ionic transference numbers, activation energy of ion transport and breakdown voltage of bilayer lipid membranes made from dioleoylphosphatidylcholine or its mixtures with dolichyl 12-phosphate have been studied. The electrical measurements showed that dolichyl phosphate in phospholipid bilayers decreases membrane permeability, changes membrane ionic selectivity and increases membrane stability. These results are discussed in light of the aggregation behavior and the intramolecular clustering of a dolichyl phosphate molecule in phospholipid membranes. From our data we suggest that the hydrophilic part of dolichyl phosphate molecules regulates their behavior in membranes.

摘要

研究了由二油酰磷脂酰胆碱或其与十二烷基磷酸酯的混合物制成的双层脂质膜的离子渗透系数、离子迁移数、离子运输活化能和击穿电压。电学测量表明，磷脂双层中的磷酸十二烷基酯降低了膜通透性，改变了膜离子选择性，并提高了膜稳定性。根据磷酸十二烷基酯分子在磷脂膜中的聚集行为和分子内聚集情况对这些结果进行了讨论。根据我们的数据，我们认为磷酸十二烷基酯分子的亲水部分调节了它们在膜中的行为。