State Key Laboratory of Veterinary Etiological Biology; Key Laboratory of Veterinary Parasitology of Gansu Province; Innovation of Research Program of Gastrointestinal Infection and Mucosal Immunity of Poultry and Pig; Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, People's Republic of China.
Jiangsu Co-Innovation Center for Prevention and Control of Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu Province 225009, People's Republic of China.
J Proteome Res. 2023 Sep 1;22(9):2785-2802. doi: 10.1021/acs.jproteome.2c00726. Epub 2023 Aug 10.
Proteome-wide lysine acetylation has been documented in apicomplexan parasite and . Here, we conducted the first lysine acetylome in unsporulated oocysts (USO), sporulated 7 h oocysts (SO 7h), sporulated oocysts (SO), sporozoites (S), and the second generation merozoites (SMG) of through a 4D label-free quantitative technique. Altogether, 8532 lysine acetylation sites on 2325 proteins were identified in , among which 5445 sites on 1493 proteins were quantified. In addition, 557, 339, 478, 248, 241, and 424 differentially expressed proteins were identified in the comparisons SO7h vs USO, SO vs SO7h, SO vs USO, S vs SO, SMG vs S, and USO vs SMG, respectively. The bioinformatics analysis of the acetylome showed that the lysine acetylation is widespread on proteins of diverse functions. Moreover, the dynamic changes of lysine acetylome among different life stages revealed significant regulation during the whole process of growth and stage conversion. This study provides a beginning for the investigation of the regulate role of lysine acetylation in and may provide new strategies for anticoccidiosis drug and vaccine development. Raw data are publicly available at iProX with the data set identifier PXD040368.
蛋白质组范围内的赖氨酸乙酰化在顶复门寄生虫 和 中已有记载。在这里，我们通过无标记 4D 定量技术，首次对未孢子化卵囊（USO）、孢子化 7 小时卵囊（SO 7h）、孢子化卵囊（SO）、裂殖子（S）和第二代裂殖子（SMG）中的 进行了赖氨酸乙酰组学研究。总共在 中鉴定到 2325 种蛋白质上的 8532 个赖氨酸乙酰化位点，其中 1493 种蛋白质上的 5445 个位点被定量。此外，在 SO7h 与 USO、SO 与 SO7h、SO 与 USO、S 与 SO、SMG 与 S 以及 USO 与 SMG 的比较中，分别鉴定到 557、339、478、248、241 和 424 个差异表达蛋白。乙酰组学的生物信息学分析表明，赖氨酸乙酰化广泛存在于具有不同功能的蛋白质上。此外，不同生活阶段之间赖氨酸乙酰组的动态变化表明，在 的整个生长和阶段转换过程中存在显著的调控。本研究为研究赖氨酸乙酰化在 中的调控作用提供了一个开端，可能为抗球虫药物和疫苗的开发提供新的策略。原始数据可在 iProX 上公开获取，数据集标识符为 PXD040368。
