脱氧核糖核酸酶I对肌肉原肌球蛋白聚合的影响。

Effect of DNAase I on muscle tropomyosin polymerization.

作者信息

Payne M R, Badoyannis H, Rudnick S E

出版信息

Biochim Biophys Acta. 1986 Oct 1;883(3):454-9. doi: 10.1016/0304-4165(86)90284-9.

Abstract

DNAase I, an endonuclease which interacts with G-actin, also affects tropomyosin polymerization. With chicken pectoralis or bovine cardiac ventricle tropomyosin, DNAase I both prevents tropomyosin from polymerizing and disrupts already formed tropomysin filaments. DNAase I and filament tropomyosin can also form a precipitable complex. In the electron microscope, the complex is observed as irregularly margined stellate-shaped structures with a maximum size of 9 micron. Isolated DNAase I-tropomyosin stellate complex consists of a 2:1 molar ratio of DNAase I and tropomyosin, suggesting that each tropomyosin subunit can bind DNAase I.

摘要

脱氧核糖核酸酶I是一种能与G-肌动蛋白相互作用的核酸内切酶，它也会影响原肌球蛋白的聚合。对于鸡胸肌或牛心室原肌球蛋白，脱氧核糖核酸酶I既能阻止原肌球蛋白聚合，又能破坏已形成的原肌球蛋白丝。脱氧核糖核酸酶I和丝状原肌球蛋白也能形成可沉淀的复合物。在电子显微镜下，该复合物呈现为边缘不规则的星状结构，最大尺寸为9微米。分离出的脱氧核糖核酸酶I-原肌球蛋白星状复合物由脱氧核糖核酸酶I和原肌球蛋白以2:1的摩尔比组成，这表明每个原肌球蛋白亚基都能结合脱氧核糖核酸酶I。