Institute for Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210023, China; Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, 210037, China.
Institute for Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210023, China.
Environ Pollut. 2023 Oct 15;335:122337. doi: 10.1016/j.envpol.2023.122337. Epub 2023 Aug 8.
Plant roots continuously influence the rhizosphere, which also serves as a recruitment site for microorganisms with desirable functions. The development of genetically engineered (GE) crop varieties has offered unparalleled yield advantages. However, in-depth research on the effects of GE crops on the rhizosphere microbiome is currently insufficient. We used a triple-transgenic soybean cultivar (JD606) that is resistant to insects, glyphosate, and drought, along with its control, ZP661, and JD606 treated with glyphosate (JD606G). Using 16S and ITS rDNA sequencing, their effects on the taxonomy and function of the bacterial and fungal communities in the rhizosphere, surrounding, and bulk soil compartment niches were determined. Alpha diversity demonstrated a strong influence of JD606 and JD606G on bacterial Shannon diversity. Both treatments significantly altered the soil's pH and nitrogen content. Beta diversity identified the soil compartment niche as a key factor with a significant probability of influencing the bacterial and fungal communities associated with soybeans. Further analysis showed that the rhizosphere effect had a considerable impact on bacterial communities in JD606 and JD606G soils but not on fungal communities. Microbacterium, Bradyrhizobium, and Chryseobacterium were found as key rhizobacterial nodes. In addition, the LEfSe analysis identified biomarker taxa with plant-beneficial attributes, demonstrating rhizosphere-driven microbial recruitment. FUNGuild, Bugbase, and FAPROTAX functional predictions showed that ZP661 soils had more plant pathogen-associated microbes, while JD606 and JD606G soils had more stress-tolerance, nitrogen, and carbon cycle-related microbes. Bacterial rhizosphere networks had more intricate topologies than fungal networks. Furthermore, correlation analysis revealed that the bacteria and fungi with higher abundances exhibited varying degrees of positive and negative correlations. Our findings shed new light on the niche partitioning of bacterial and fungal communities in soil. It also indicates that following triple-transgenic soybean cultivation and glyphosate application, plant roots recruit microbes with beneficial taxonomic and functional traits in the rhizosphere.
植物根系不断影响根际，根际也是具有理想功能的微生物的招募地。转基因（GE）作物品种的发展带来了无与伦比的产量优势。然而，目前对 GE 作物对根际微生物组影响的深入研究还不够。我们使用了一种抗虫、抗草甘膦和耐旱的三重转基因大豆品种（JD606），及其对照品种 ZP661 和用草甘膦处理的 JD606（JD606G）。通过 16S 和 ITS rDNA 测序，确定了它们对根际、周围和土壤本体土壤分室中细菌和真菌群落的分类和功能的影响。α多样性表明 JD606 和 JD606G 对细菌 Shannon 多样性有很强的影响。两种处理都显著改变了土壤的 pH 值和氮含量。β多样性确定土壤分室是影响与大豆相关的细菌和真菌群落的关键因素，具有显著的概率。进一步分析表明，根际效应对 JD606 和 JD606G 土壤中的细菌群落有很大影响，但对真菌群落没有影响。发现 Microbacterium、Bradyrhizobium 和 Chryseobacterium 是关键的根际细菌节点。此外，LEfSe 分析确定了具有植物有益属性的生物标志物分类群，表明根际驱动的微生物招募。FUNGuild、Bugbase 和 FAPROTAX 功能预测表明，ZP661 土壤中与植物病原体相关的微生物较多，而 JD606 和 JD606G 土壤中具有更多的抗逆性、氮和碳循环相关的微生物。细菌根际网络具有更复杂的拓扑结构。此外，相关分析表明，丰度较高的细菌和真菌表现出不同程度的正相关和负相关。我们的研究结果揭示了土壤中细菌和真菌群落的生态位划分。它还表明，在三重转基因大豆种植和草甘膦应用后，植物根系在根际招募具有有益分类和功能特征的微生物。
