Laboratoire de Physiologie Écologique et Évolutive Marine, Département de Biologie, Chimie et Géographie, Université du Québec à Rimouski, Rimouski, Canada.
Department of Biological Sciences, University of Manitoba, Winnipeg, Canada.
J Comp Physiol B. 2023 Oct;193(5):509-522. doi: 10.1007/s00360-023-01507-3. Epub 2023 Aug 10.
Crustaceans' endocrinology is a vastly understudied area of research. The major focus of the studies on this topic to date has been on the molting cycle (and in particular, the role of crustacean hyperglycemic hormone (CHH)), as well as the role of other hormones in facilitating physiological phenotypic adjustments to salinity changes. Additionally, while many recent studies have been conducted on the acclimation and adaptation capacity of crustaceans to a changing environment, only few have investigated internal hormonal balance especially with respect to an endocrine response to environmental challenges. Consequently, our study aimed to identify and characterize endocrine components of acid-base regulation in the European green crab, Carcinus maenas. We show that both the biogenic amine octopamine (OCT) and the CHH are regulatory components of branchial acid-base regulation. While OCT suppressed branchial proton excretion, CHH seemed to promote it. Both hormones were also capable of enhancing branchial ammonia excretion. Furthermore, mRNA abundance for branchial receptors (OCT-R), or G-protein receptor activated soluble guanylate cyclase (sGC1b), are affected by environmental change such as elevated pCO (hypercapnia) and high environmental ammonia (HEA). Our findings support a role for both OCT and CHH in the general maintenance of steady-state acid-base maintenance in the gill, as well as regulating the acid-base response to environmental challenges that C. maenas encounters on a regular basis in the habitats it dwells in and more so in the future ocean.
甲壳动物内分泌学是一个研究得非常不充分的领域。迄今为止，该主题研究的主要重点一直是蜕皮周期（特别是甲壳动物高血糖激素 (CHH) 的作用），以及其他激素在促进对盐度变化的生理表型调整中的作用。此外，虽然最近有许多研究针对甲壳动物对不断变化的环境的适应和适应能力，但只有少数研究调查了内部激素平衡，特别是针对内分泌对环境挑战的反应。因此，我们的研究旨在确定和描述欧洲绿蟹（Carcinus maenas）酸碱调节的内分泌成分。我们表明，生物胺章鱼胺 (OCT) 和 CHH 都是鳃酸碱调节的调节成分。虽然 OCT 抑制鳃质子排泄，但 CHH 似乎促进了它。这两种激素也能够增强鳃氨排泄。此外，环境变化（如高二氧化碳分压（高碳酸血症）和高环境氨（HEA））会影响鳃受体（OCT-R）或 G 蛋白受体激活可溶性鸟苷酸环化酶（sGC1b）的 mRNA 丰度。我们的发现支持 OCT 和 CHH 在鳃中稳态酸碱维持以及调节甲壳动物在其栖息地经常遇到的环境挑战的酸碱反应中的一般作用，尤其是在未来的海洋中。
