Schmid Tristan, Wegener Florian, Hotfiel Thilo, Hoppe Matthias W
Movement and Training Science, Leipzig University, Jahnallee 59, 04109, Leipzig, Germany.
Center for Musculoskeletal Surgery Osnabrück (OZMC), Klinikum Osnabrück, Am Finkenhügel 1, 49076, Osnabrueck, Germany.
J Exp Orthop. 2023 Aug 10;10(1):81. doi: 10.1186/s40634-023-00645-5.
The aim of this systematic review was to investigate tendon-specific microRNAs (miRNAs) as biomarkers for the detection of tendinopathies or degenerative tendon ruptures. Also, their regulatory mechanisms within the tendon pathophysiology were summarized.
A systematic literature research was performed using the PRISMA guidelines. The search was conducted in the Pubmed database. The SIGN checklist was used to assess the study quality of the included original studies. To determine the evidence and direction of the miRNA expression rates, a best-evidence synthesis was carried out, whereby only studies with at least a borderline methodological quality were considered for validity purposes.
Three thousand three hundred seventy studies were reviewed from which 22 fulfilled the inclusion criteria. Moderate evidence was found for miR-140-3p and miR-425-5p as potential biomarkers for tendinopathies as well as for miR-25-3p, miR-29a-3p, miR-140-3p, and miR-425-5p for the detection of degenerative tendon ruptures. This evidence applies to tendons at the upper extremity in elderly patients. All miRNAs were associated with inflammatory cytokines as interleukin-6 or interleukin-1ß and tumor necrosis factor alpha.
Moderate evidence exists for four miRNAs as potential biomarkers for tendinopathies and degenerative tendon ruptures at the upper extremity in elderly patients. The identified miRNAs are associated with inflammatory processes.
本系统评价旨在研究肌腱特异性微小RNA（miRNA）作为检测肌腱病或退行性肌腱断裂生物标志物的情况。此外，还总结了它们在肌腱病理生理学中的调控机制。
按照PRISMA指南进行系统的文献检索。检索在PubMed数据库中进行。使用SIGN清单评估纳入的原始研究的质量。为了确定miRNA表达率的证据和方向，进行了最佳证据综合分析，即仅考虑方法学质量至少达到临界水平的研究以确保有效性。
共检索了3370项研究，其中22项符合纳入标准。发现miR-140-3p和miR-425-5p有中等证据可作为肌腱病的潜在生物标志物，miR-25-3p、miR-29a-3p、miR-140-3p和miR-425-5p有中等证据可用于检测退行性肌腱断裂。该证据适用于老年患者上肢的肌腱。所有miRNA均与白细胞介素-6或白细胞介素-1β以及肿瘤坏死因子α等炎性细胞因子相关。
有中等证据表明，四种miRNA可作为老年患者上肢肌腱病和退行性肌腱断裂的潜在生物标志物。所鉴定的miRNA与炎症过程相关。
