Mo Guichun, Qin Dongmiao, Wu Yusheng, Luo Zhi, Mo Keting, Deng Biyang
State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin, 541004, China.
Guangxi Key Lab of Agricultural Resources Chemistry and Biotechnology, School of Chemistry and Food Science, Yulin Normal University, Yulin, 537000, China.
Mikrochim Acta. 2023 Aug 11;190(9):347. doi: 10.1007/s00604-023-05934-5.
A new electrochemiluminescence (ECL) cytosensor is proposed for the simultaneous determination of phosphatidylserine (PS) and epidermal growth factor receptor (EGFR) based on the ECL signals of metal-organic framework-5 (MOF-5) loaded CdS quantum dots and N-(aminobutyl)-N-(ethylisoluminol)-polyethylenimine capped Au and Ag nanoparticles. Apoptosis promotes the exposure of PS and reduces the expression of EGFR in cell membranes. Two spatially resolved areas on dual-disk glassy carbon electrodes were designed to eliminate the interference from different ECL probes. Using HepG2 cells treated with resveratrol to induce apoptosis, the cytosensor exhibited high sensitivity, simplicity, and high reproducibility, demonstrating its potential in drug screening and rapid apoptotic cell detection. The strategy reported provides a promising platform for the highly sensitive cytosensing and convenient screening of clinically relevant anticancer drugs.
基于负载金属有机框架-5（MOF-5）的硫化镉量子点以及N-(氨丁基)-N-(乙基异鲁米诺)-聚乙烯亚胺包覆的金和银纳米颗粒的电化学发光（ECL）信号，提出了一种用于同时测定磷脂酰丝氨酸（PS）和表皮生长因子受体（EGFR）的新型ECL细胞传感器。细胞凋亡会促使PS暴露，并降低细胞膜中EGFR的表达。在双盘玻碳电极上设计了两个空间分辨区域，以消除不同ECL探针之间的干扰。使用经白藜芦醇处理诱导凋亡的肝癌细胞系HepG2细胞，该细胞传感器表现出高灵敏度、简便性和高重现性，证明了其在药物筛选和快速凋亡细胞检测中的潜力。所报道的策略为高灵敏度细胞传感和方便地筛选临床相关抗癌药物提供了一个有前景的平台。
