State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin, 541004, China.
State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin, 541004, China.
Biosens Bioelectron. 2021 Apr 15;178:113024. doi: 10.1016/j.bios.2021.113024. Epub 2021 Jan 21.
A new electrochemiluminescence (ECL) immunosensor based on spatially-resolved dual-potential technology was designed for the simultaneous determination of carbohydrate antigen 19-9 (CA 19-9) and carbohydrate antigen 24-2 (CA 242). Luminol-AgNPs@ZIF-67 was used as the anodic probe, and Pt nanoparticle-functionalized graphitic carbon nitride nanosheets (g-CN@PtNPs) were used as the cathodic probe. Two spatially-resolved areas on the dual-disk glassy carbon electrode (DDGCE) were modified with a AuNPs film by electrodeposition to improve the conductivity of the sensing interface. By recording the ECL responses at two different excitation potentials, the linear range for CA 19-9 was determined to be 0.0001-10 U/mL, with a limit of detection of 31 μU/mL. The linear range for CA 242 was 0.0005-10 U/mL, with a limit of detection of 0.16 mU/mL. Moreover, the ECL immunosensor possessed high selectivity and stability and successfully detected CA 19-9 and CA 242 in real samples. This immunosensor provides a new platform for clinical immunoassays.
基于空间分辨双电位技术的新型电化学发光（ECL）免疫传感器被设计用于同时测定糖抗原 19-9（CA 19-9）和糖抗原 24-2（CA 242）。将纳米银包裹的鲁米诺（Luminol-AgNPs@ZIF-67）用作阳极探针，Pt 纳米粒子功能化的石墨相氮化碳纳米片（g-CN@PtNPs）用作阴极探针。通过电沉积在双盘玻碳电极（DDGCE）的两个空间分辨区域上修饰 AuNPs 薄膜，以提高传感界面的导电性。通过在两个不同的激发电位下记录 ECL 响应，确定 CA 19-9 的线性范围为 0.0001-10 U/mL，检测限为 31 μU/mL。CA 242 的线性范围为 0.0005-10 U/mL，检测限为 0.16 mU/mL。此外，该 ECL 免疫传感器具有高选择性和稳定性，并成功地检测了真实样品中的 CA 19-9 和 CA 242。该免疫传感器为临床免疫分析提供了一个新的平台。
