Novosibirsk Research Institute of Tuberculosis, Ministry of Health of the Russian Federation, Novosibirsk, Russia.
Novosibirsk State University, Novosibirsk, Russia.
Bull Exp Biol Med. 2023 Jul;175(3):367-370. doi: 10.1007/s10517-023-05869-0. Epub 2023 Aug 10.
In a series of in vitro experiments, the optimum regimes of laser treatment were determined for effective photodynamic inactivation of Mycobacterium tuberculosis at a constant dose of aluminum phthalocyanine. Reference laboratory drug-susceptible strain H37Rv and clinical isolates of M. tuberculosis with varying degrees of resistance to antibiotics were used. Suspensions of M. tuberculosis were incubated with aluminum phthalocyanine in a concentration of 5 μg/ml and then subjected to photodynamic inactivation with high- or low- intensity laser irradiation at λ=662 nm at various parameters of light power density. Mycobacteria survival rate was assessed by CFU assay on solid media. It was shown that at the specified dose of the photosensitizer, the photodynamic inactivation of mycobacterium was characterized by inhibition and complete cessation of their growth depending on the dose density of the laser energy. Effective photodynamic inactivation started from a light dose density of 46.9 J/cm at a radiation power of 0.01 W and from 56.25 J/cm at a radiation power of 0.1 W. Photodynamic inactivation at low laser power is more effective against drug-susceptible strains of M. tuberculosis.
在一系列体外实验中，针对在铝酞菁染料的恒定剂量下有效光动力灭活结核分枝杆菌，确定了激光治疗的最佳方案。使用参考实验室药敏株 H37Rv 和具有不同程度抗生素耐药性的结核分枝杆菌临床分离株。将结核分枝杆菌悬浮液在 5μg/ml 的浓度下与铝酞菁染料孵育，然后用 λ=662nm 的高强度或低强度激光照射进行光动力灭活，光功率密度的各种参数不同。通过固体培养基上的 CFU 测定评估分枝杆菌的存活率。结果表明，在规定的光敏剂剂量下，光动力灭活结核分枝杆菌的特征是根据激光能量的剂量密度抑制和完全停止其生长。有效光动力灭活从辐射功率为 0.01W 时的 46.9J/cm 的光剂量密度开始，从辐射功率为 0.1W 时的 56.25J/cm 的光剂量密度开始。低激光功率的光动力灭活对结核分枝杆菌的药敏株更有效。
