Department of Agriculture - Agricultural Research Service, United States, San Joaquin Valley Agricultural Sciences Center, Parlier, CA, 93648, USA.
BMC Res Notes. 2023 Aug 10;16(1):166. doi: 10.1186/s13104-023-06443-7.
Citrus yellow vein clearing virus (CYVCV) is an emerging disease that poses a significant threat to the citrus industry in California. In this study, the viral genomic RNA was isolated from Eureka lemon plants in the greenhouse exhibiting CYVCV symptoms. Subsequently, the corresponding DNA genome amplicon was sequenced and annotated. These efforts expand the genotype database of CYVCV, which aims to enhance detection assays, promote understanding of the virus's genetics and evolution, and support the management of this disease.
In this report, we present the complete genome sequence of the CYVCV California isolate (CA1). The genome was found to be 7,530 bp in length, with a G + C content of 51.7%. The 5' and 3' termini were determined using 5' and 3' termini rapid amplification of cDNA ends (RACE) systems. Furthermore, our analysis revealed the presence of six open reading frames (ORFs) potentially encoding proteins. All sequence data and annotation have been deposited in GenBank under the accession number OR037276.1.
柑橘黄龙病清除病毒（CYVCV）是一种新兴疾病，对加利福尼亚州的柑橘产业构成重大威胁。在这项研究中，从温室中表现出 CYVCV 症状的尤力克柠檬植株中分离出病毒基因组 RNA。随后，对相应的 DNA 基因组扩增子进行了测序和注释。这些努力扩展了 CYVCV 的基因型数据库，旨在增强检测方法、促进对病毒遗传学和进化的理解，并支持该疾病的管理。
在本报告中，我们介绍了加利福尼亚分离株（CA1）的 CYVCV 全基因组序列。基因组全长 7530bp，G+C 含量为 51.7%。使用 5'和 3'末端快速扩增 cDNA 末端（RACE）系统确定了 5'和 3'末端。此外，我们的分析还揭示了存在六个潜在编码蛋白质的开放阅读框（ORFs）。所有序列数据和注释均已在 GenBank 中以 OR037276.1 的登录号进行了存储。
