Liao Ping, Dan Xue, Ge Wen, Zhang Qi, Zhao Jinfa, Zhou Changyong, Zhou Yan
Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Citrus Research Institute, Southwest University, Xiema Street, Beibei District, Chongqing 400712, China.
National Citrus Engineering and Technology Research Center, Citrus Research Institute, Southwest University, Xiema Street, Beibei District, Chongqing 400712, China.
Hortic Res. 2024 Oct 11;12(1):uhae287. doi: 10.1093/hr/uhae287. eCollection 2025 Jan.
Glycerophosphodiester phosphodiesterase 1 (GDPD1) plays an important function in the abiotic stress responses and participates in the accumulation of sn-glycerol-3-phosphate (G3P) in plants, which is key to plant systemic acquired resistance (SAR). However, the role of GDPD1 in plant responses to biotic stress remains poorly understood. This study characterized the antivirus function of the gene (designated as ) from Eureka lemon. ClGDPD1 is located in the membrane and endoplasmic reticulum, where it interacts with the citrus yellow vein clearing virus (CYVCV) coat protein (CP). Compared to individually expressed ClGDPD1 or coexpressed ClGDPD1 + CP, transiently coexpressed ClGDPD1 + CP or ClGDPD1 + CP significantly upregulated the key substance contents and genes expression involved in glycerophospholipid metabolism. Over-expression of significantly facilitated the accumulation of G3P, upregulated the expression of SAR-related genes, and increased the resistance of transgenic Eureka lemon to CYVCV infection. Furthermore, exogenous glycerol treatment and over-expression of increased the G3P content and reduced CYVCV titers in plants or hairy roots. These results indicated that the enhanced resistance of transgenic Eureka lemon to CYVCV may be due to facilitating G3P accumulation through the interaction of ClGDPD1 with CP. Our findings provide novel insights into the role of ClGDPD1 as an important regulatory center in mediating the citrus defense response to viral infections.
甘油磷酸二酯磷酸二酯酶1（GDPD1）在植物非生物胁迫响应中发挥重要作用，并参与植物中sn-甘油-3-磷酸（G3P）的积累，而G3P是植物系统获得性抗性（SAR）的关键。然而，GDPD1在植物对生物胁迫响应中的作用仍知之甚少。本研究对来自尤力克柠檬的该基因（命名为ClGDPD1）的抗病毒功能进行了表征。ClGDPD1定位于膜和内质网，在那里它与柑橘黄脉清病毒（CYVCV）外壳蛋白（CP）相互作用。与单独表达的ClGDPD1或共表达的ClGDPD1 + CP相比，瞬时共表达的ClGDPD1 + CP或ClGDPD1 + CP显著上调了甘油磷脂代谢相关关键物质的含量和基因表达。ClGDPD1的过表达显著促进了G3P的积累，上调了SAR相关基因的表达，并增强了转基因尤力克柠檬对CYVCV感染的抗性。此外，外源甘油处理和ClGDPD1的过表达增加了植物或毛状根中的G3P含量并降低了CYVCV滴度。这些结果表明，ClGDPD1转基因尤力克柠檬对CYVCV抗性增强可能是由于ClGDPD1与CP相互作用促进了G3P积累。我们的研究结果为ClGDPD1作为介导柑橘对病毒感染防御反应的重要调控中心的作用提供了新的见解。
