Jiang Ying, Tang Jie, Liu Xiangjian, Daroch Maurycy
School of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen, China.
School of Food and Bioengineering, Chengdu University, Chengdu, Sichuan, China.
Front Microbiol. 2023 Jul 26;14:1176500. doi: 10.3389/fmicb.2023.1176500. eCollection 2023.
Thermophilic cyanobacteria play a crucial role as primary producers in hot spring ecosystems, yet their microbiological, taxonomic, and ecological characteristics are not extensively studied. This study aimed to characterize a novel strain of thermophilic cyanobacteria, PKUAC-SCTA174 (A174), using a combination of traditional polyphasic methods and modern genomic-based approaches. The study included 16S rRNA-based phylogeny, ITS secondary structure prediction, morphological and habitat analyses, as well as high-quality genome sequencing with corresponding phylogenomic analyses. The results of the 16S rRNA, 16S-23S ITS secondary structure, morphology, and habitat analyses supported the classification of the strain as a member of a novel genus within the family , closely related to and . Genomic analysis revealed the presence of a sophisticated carbon-concentrating mechanism (CCM) in the strain, involving two CO uptake systems NDH-I, and NDH-I, three types of bicarbonate transporters (BCT1, bicA, sbtA,) and two distinct putative carboxysomal carbonic anhydrases ( and ). The expression of CCM genes was investigated with a CO shift experiment, indicating varying transcript abundance among different carbon uptake systems. Based on the comprehensive characterization, the strain was delineated as , based on the botanical code. The study of the complete genome of strain A174 contributes valuable insights into the genetic characteristics of the genus and related organisms and provides a systematic understanding of thermophilic cyanobacteria. The findings presented here offer valuable data that can be utilized for future research in taxogenomics, ecogenomics, and geogenomics.
嗜热蓝细菌作为温泉生态系统中的初级生产者发挥着关键作用，但其微生物学、分类学和生态学特征尚未得到广泛研究。本研究旨在结合传统多相方法和现代基于基因组的方法，对嗜热蓝细菌新菌株PKUAC-SCTA174（A174）进行表征。该研究包括基于16S rRNA的系统发育分析、ITS二级结构预测、形态学和栖息地分析，以及高质量基因组测序和相应的系统基因组学分析。16S rRNA、16S-23S ITS二级结构、形态学和栖息地分析结果支持将该菌株分类为 科内一个新属的成员，与 和 密切相关。基因组分析显示该菌株存在复杂的碳浓缩机制（CCM），涉及两个CO吸收系统NDH-I和NDH-I、三种类型的碳酸氢盐转运蛋白（BCT1、bicA、sbtA）和两种不同的假定羧基体碳酸酐酶（ 和 ）。通过CO转移实验研究了CCM基因的表达，表明不同碳吸收系统之间的转录本丰度不同。基于全面表征，根据植物学代码将该菌株划定为 。对菌株A174完整基因组的研究为 属及相关生物的遗传特征提供了有价值的见解，并提供了对嗜热蓝细菌的系统理解。本文的研究结果提供了可用于未来分类基因组学、生态基因组学和地质基因组学研究的宝贵数据。
