培养的震颤小鼠雪旺细胞：脂质和蛋白质合成异常。

Trembler mouse Schwann cells in culture: anomalies in the synthesis of lipids and proteins.

作者信息

White F V, Burroni D, Ceccarini C, Matthieu J M, Manetti R, Ceccarini E C

出版信息

Brain Res. 1986 Aug 27;381(1):85-92. doi: 10.1016/0006-8993(86)90693-1.

Abstract

We investigated the biochemical and growth properties of Schwann cells from the sciatic nerve of Trembler and unaffected mice in culture. Both Trembler and control cultures showed similar growth rates. The specific activity of 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP) and enzymes involved in lipid metabolism of cerebrosides and sulfatides were studied. UDP-galactose: ceramide galactosyltransferase was significantly decreased in Trembler cultures less than 21 days in vitro. No differences were found in the specific activities of cerebroside sulfotransferase, arylsulfatase A or CNP between Trembler and control cultures. Schwann cells from Trembler and control mice were labeled with [35S]methionine and the protein analyzed by two-dimensional gel electrophoresis. Our study revealed few but consistent differences in the protein pattern synthesized by the Trembler Schwann cells.

摘要

我们研究了培养的震颤小鼠和未受影响小鼠坐骨神经中雪旺细胞的生化及生长特性。震颤小鼠和对照培养物的生长速率相似。我们研究了2',3'-环核苷酸3'-磷酸二酯酶（CNP）以及参与脑苷脂和硫脂脂质代谢的酶的比活性。在体外培养少于21天的震颤小鼠培养物中，UDP-半乳糖：神经酰胺半乳糖基转移酶显著降低。震颤小鼠和对照培养物之间，脑苷脂硫酸转移酶、芳基硫酸酯酶A或CNP的比活性未发现差异。用[35S]甲硫氨酸标记震颤小鼠和对照小鼠的雪旺细胞，并通过二维凝胶电泳分析蛋白质。我们的研究揭示，震颤小鼠雪旺细胞合成的蛋白质图谱中存在一些虽少但一致的差异。