Lipid metabolism in peripheral nerve cell culture (rich in Schwann cells) from normal and trembler mice.

A culture of peripheral nerve cells, very rich in Schwann cells, was developed from sciatic nerve. In both normal and Trembler, typical spindle-shaped cells were seen; most of the cells were surrounded by basement membrane-like material (predominantly in-between adjacent cells). In Trembler cells, cultivated in the presence of labelled acetate, the fatty acids were slightly altered; phosphatidylcholine was slightly reduced and phosphatidylethanolamine increased. Sulfatides were increased four times.