Uphouse L, Williams J, Eckols K, Sierra V
Brain Res. 1986 Sep 3;381(2):376-81. doi: 10.1016/0006-8993(86)90093-4.
The binding of [3H]5-hydroxytryptamine ([3H]5-HT) to cortical membranes was examined in female rats during diestrus, proestrus and estrus. Serotonin binding was lowest during the early afternoon of proestrus and highest during the afternoon of estrus with diestrous values in between. The high estrous values were associated with a significant increase in Bmax and a decrease in Kd. However, the increase in binding actually took place during the late afternoon of proestrus, when there was a rapid increase in binding from noon to 18.00 h. Binding remained at this elevated level throughout the next day. There were no changes in the binding of [3H]ketanserin or [3H]spiroperidol to cortical membranes during the estrous cycle, so the differential binding of [3H]5-HT is most likely the result of variations in 5-HT1 rather than 5-HT2 receptors. These observations of changes in serotonin binding in a brain area nearly devoid of sex steroid receptors suggest that the hormonal fluctuations accompanying the female estrous cycle influence brain areas other than those classically thought to regulate neuroendocrine function.
在雌性大鼠的动情间期、动情前期和动情期，研究了[3H]5-羟色胺（[3H]5-HT）与皮质膜的结合情况。血清素结合在动情前期午后早期最低，在动情期午后最高，动情间期的值介于两者之间。动情期的最高值与Bmax显著增加和Kd降低有关。然而，结合的增加实际上发生在动情前期午后较晚的时候，即从中午到18:00时结合迅速增加。在第二天，结合一直保持在这个升高的水平。在动情周期中，[3H]酮色林或[3H]螺哌啶与皮质膜的结合没有变化，因此[3H]5-HT的差异结合很可能是5-HT1而非5-HT2受体变化的结果。在一个几乎没有性类固醇受体的脑区观察到血清素结合的变化，这些观察结果表明，伴随雌性动情周期的激素波动会影响除经典认为调节神经内分泌功能的脑区之外的其他脑区。
