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颈动脉体球细胞的细胞内钾活性、钾平衡电位和膜电位。

Intracellular potassium activity, potassium equilibrium potential and membrane potential of carotid body glomus cells.

作者信息

Oyama Y, Walker J L, Eyzaguirre C

出版信息

Brain Res. 1986 Sep 3;381(2):405-8. doi: 10.1016/0006-8993(86)90100-9.

DOI:10.1016/0006-8993(86)90100-9
PMID:3756516
Abstract

The intracellular potassium activity (ai(K)) of glomus cells in isolated rabbit carotid bodies was measured with potassium ion selective microelectrodes (K+ electrodes). The measurements yielded a mean ai(K) value of 33.0 mM in Hepes-buffered Tyrode's solution equilibrated with 100% O2. This value is significantly higher than that predicted by assuming that K+ ions are passively distributed across the membrane of glomus cells. The relationship between the membrane potential (EM) and the equilibrium potential of K+ ions (EK) at various extracellular potassium activities (ao(K] suggests that K+ ions are not involved in the maintenance of the carotid body glomus cell EM.

摘要

采用钾离子选择性微电极(K⁺电极)测量了离体兔颈动脉体球细胞的细胞内钾活性(ai(K))。在与100% O₂平衡的Hepes缓冲台氏液中进行测量,得到的ai(K)平均值为33.0 mM。该值显著高于假设K⁺离子在球细胞膜上被动分布所预测的值。在不同细胞外钾活性(ao(K))下,膜电位(EM)与K⁺离子平衡电位(EK)之间的关系表明,K⁺离子不参与颈动脉体球细胞膜电位的维持。

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