离体兔颈动脉体中球细胞的细胞内氯离子活性

The intracellular chloride activity of glomus cells in the isolated rabbit carotid body.

作者信息

Oyama Y, Walker J L, Eyzaguirre C

出版信息

Brain Res. 1986 Mar 12;368(1):167-9. doi: 10.1016/0006-8993(86)91056-5.

Abstract

The resting membrane potential (EM) and intracellular chloride activity (ai(CI] of glomus cells were measured in isolated rabbit carotid bodies using conventional and chloride-selective microelectrodes. EM and ai(CI) were -25.9 +/- 1.4 mV (mean +/- S.E.M., n = 13) and 20.9 +/- 1.9 mV (mean +/- S.E.M., n = 13), respectively. When the chloride equilibrium potential (ECI) was shifted in a positive direction, EM depolarized. It is concluded that chloride ions are not passively distributed across the glomus cell membrane and that they play an important role in determining EM.

摘要

使用传统微电极和氯离子选择性微电极，在分离的兔颈动脉体中测量了球细胞的静息膜电位（EM）和细胞内氯离子活性（ai[CI]）。EM和ai(CI)分别为-25.9±1.4mV（平均值±标准误，n = 13）和20.9±1.9mV（平均值±标准误，n = 13）。当氯离子平衡电位（ECI）向正向移动时，EM去极化。得出的结论是，氯离子不是被动地分布在球细胞膜上，并且它们在决定EM方面起着重要作用。