Thiemann W, Malisch R, Reymann K G
Brain Res Bull. 1986 Jul;17(1):1-4. doi: 10.1016/0361-9230(86)90154-1.
The construction of a simple universal chamber for long-term recording from submerged and interface brain slices is described. The medium is circulated and oxygenated constantly by an O2-CO2 gas mixture using the principle of a bubble pump. The design permits experiments which require long-term exposure to expensive drugs as well as great economy of oxygen consumption.