Department of Animal Molecular Biology, National Research Institute of Animal Production, Balice, Poland.
Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Torun, Torun, Poland.
Equine Vet J. 2024 May;56(3):586-597. doi: 10.1111/evj.13984. Epub 2023 Aug 10.
Recent studies have shown that in Arabian horse muscle, long-term exercise-induced expression of genes related to fatty acid degradation and the downregulation of genes belonging to the glycolysis/gluconeogenesis and insulin signalling pathways. Long-lasting physical exertion may trigger the metabolism to switch the main energy source from carbohydrates to lipids due to higher caloric content.
To describe the metabolism adaptation at the whole transcriptome of blood to endurance effort in Arabian horses.
In vivo experiment.
Venous blood samples from 10 Arabian horses were taken before and after a 120 km long endurance ride to isolate the RNA and perform the high-throughput NGS transcriptome sequencing.
The results, including KEGG (Kyoto Encyclopaedia of Genes and Genomes) and GO (Gene Ontology) analyses, allowed us to describe the most significantly upregulated-ARV1, DGAT2, LIPE, APOA2, MOGAT1, MOGAT2, GYS1, GYS2 and downregulated-ACACA, ACACB, FADS1, FADS2 genes involved in carbohydrate and lipid metabolism. Also, the increased expression of RAF1, KRAS and NRAS genes involved in the Insulin pathway and PI3K-Akt was shown.
Limited sample size, Arabians used for endurance racing were not compared to Arabians from other equestrian disciplines.
This general insight into the processes described supports the thesis of the lipid-carbohydrates metabolism switch in endurance Arabian horses and provides the basis for further research.
最近的研究表明，在阿拉伯马的肌肉中，长期运动诱导与脂肪酸降解相关的基因表达，并下调属于糖酵解/糖异生和胰岛素信号通路的基因。长时间的身体活动可能会由于更高的热量含量而促使新陈代谢从碳水化合物切换到脂质作为主要能量来源。
描述阿拉伯马血液在整个转录组中对耐力运动的代谢适应。
体内实验。
从 10 匹阿拉伯马在 120 公里长的耐力骑行前后采集静脉血样，以分离 RNA 并进行高通量 NGS 转录组测序。
包括 KEGG（京都基因与基因组百科全书）和 GO（基因本体论）分析的结果，使我们能够描述碳水化合物和脂质代谢中上调最显著的-ARV1、DGAT2、LIPE、APOA2、MOGAT1、MOGAT2、GYS1、GYS2 和下调的-ACACA、ACACB、FADS1、FADS2 基因。还显示了胰岛素途径和 PI3K-Akt 中上调的 RAF1、KRAS 和 NRAS 基因的表达。
样本量有限，用于耐力赛的阿拉伯马与其他马术学科的阿拉伯马没有进行比较。
对所描述过程的总体了解支持了耐力阿拉伯马中脂质-碳水化合物代谢转换的论点，并为进一步研究提供了基础。
