Bogart J P, Licht L E
Can J Genet Cytol. 1986 Aug;28(4):605-17. doi: 10.1139/g86-089.
Eggs and larvae produced by diploid, triploid, and tetraploid females collected from breeding ponds on Pelee Island in Lake Erie were studied to examine the reproductive mechanism. No instance of parthenogenesis was found as all examined females required sperm to produce viable progeny. Diploid females produced diploid and triploid larvae, triploid females produced triploid and tetraploid larvae, and tetraploid females produced triploid and tetraploid larvae. The majority of the eggs produced by hybrid females do not develop or do not complete embryogenesis. Electrophoretic examination of females and their offspring demonstrate that the male genome is being incorporated in reduced as well as unreduced eggs produced by all three ploidy classes of females. The elevation of ploidy among Pelee Island Ambystoma is attributed to sperm incorporation in unreduced eggs. Triploid as well as tetraploid individuals are constantly being produced. A critical examination of the literature on parthenogenetic or gynogenetic modes of reproduction in North America Ambystoma hybrids shows no conclusive evidence supporting these modes and it is suggested that the reproductive mechanism found among Pelee Island female hybrids may be more generally applied to other hybrid Ambystoma populations.
对从伊利湖皮利岛养殖池塘收集的二倍体、三倍体和四倍体雌性蝾螈所产的卵和幼体进行了研究，以考察其繁殖机制。未发现孤雌生殖的情况，因为所有接受检查的雌性蝾螈都需要精子才能产生有活力的后代。二倍体雌性蝾螈产出二倍体和三倍体幼体，三倍体雌性蝾螈产出三倍体和四倍体幼体，四倍体雌性蝾螈产出三倍体和四倍体幼体。杂交雌性蝾螈所产的大多数卵不会发育或无法完成胚胎发育。对雌性蝾螈及其后代进行的电泳检查表明，雄性基因组被整合到了所有三种倍性类别的雌性蝾螈所产的未减数以及减数的卵中。皮利岛美西螈的倍性升高归因于未减数卵中精子的整合。三倍体以及四倍体个体不断产生。对北美美西螈杂交种孤雌生殖或雌核生殖繁殖模式的文献进行的批判性审视表明，没有确凿证据支持这些模式，有人认为在皮利岛雌性杂交种中发现的繁殖机制可能更普遍地适用于其他美西螈杂交种群。
