Biomolecular Nanoscale Engineering Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Campusvej 55, Odense 5230, Denmark.
Chem Commun (Camb). 2023 Aug 29;59(70):10548-10551. doi: 10.1039/d3cc02793g.
Assembly and fusion between different populations of lipid nanoparticles was mediated by membrane-anchored lipidated nucleic acid (LiNA) strands and observed using surface plasmon resonance (SPR) as a label-free real-time assay. Irreversible membrane fusion was distinguished from reversible assembly by enzymatical cleavage of dsDNA tethers . The assay enables user-friendly monitoring and application of membrane fusion in the context of liposomal drug delivery or synthetic biology.
通过膜锚定的脂质化核酸（LiNA）链介导不同群体的脂质纳米颗粒的组装和融合，并使用表面等离子体共振（SPR）作为无标记实时分析进行观察。通过酶切双链 DNA 连接物来区分不可逆的膜融合和可逆的组装。该测定法可方便用户监测和应用脂质体药物递送或合成生物学中膜融合的情况。
