US Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center (BARC), Beltsville, MD 20705, USA.
Department of Animal Science, Purdue University, West Lafayette, IN 47907, USA.
Biol Open. 2023 Aug 15;12(8). doi: 10.1242/bio.060044. Epub 2023 Aug 11.
Low colostrum intake relates to poorer health and infertility in swine. We previously connected vaginal lipid profiles at weaning to fertility of sows. We hypothesized vaginal lipidome varied with colostrum intake. Our objective was to determine whether indicators of colostrum intake, immunocrit (IM) and weight gain 24 h postnatal (PN), related to vaginal lipids at d21 PN. Gilts (n=60) were weighed and blood sampled to measure IM. On d21 PN vaginal swabs were taken and lipids measured using multiple reaction monitoring. Abundance of multiple lipids differed (P<0.05) between gilts categorized as high versus low IM and high versus low 24 h gain. The abundance of multiple lipids correlated with IM and 24 h gain. Phosphatidylcholine PC(36:3), PC(36:2), and arachidonic acid (C20:4) positively (P<0.05) correlated with IM. The ether lipid PCo(38:6) and multiple cholesteryl esters negatively (P<0.05) correlated with IM. ROC analysis indicated arachidonic acid and docosanoic acid (C22:0) may serve as excellent biomarkers that distinguish between high and low IM. Similar to gilts found to be infertile, lipid profiles of low colostrum intake animals had greater abundance of very long chain fatty acids, lipids with high levels of unsaturation, and cholesteryl esters, which are metabolized in peroxisomes indicating their potential dysfunction.
初乳摄入不足与猪的健康状况和不孕有关。我们之前将断奶时的阴道脂质谱与母猪的繁殖力联系起来。我们假设阴道脂质组随着初乳的摄入而变化。我们的目的是确定初乳摄入的指标（免疫球蛋白（IM）和产后 24 小时体重增加（PN））是否与产后 21 天（PN）的阴道脂质有关。小母猪（n=60）称重并采血以测量 IM。在产后 21 天 PN 时，取阴道拭子并使用多重反应监测法测量脂质。分类为高 IM 和低 IM 以及高 24 小时增重和低 24 小时增重的小母猪之间，多种脂质的丰度存在差异（P<0.05）。多种脂质的丰度与 IM 和 24 小时增重相关。磷脂酰胆碱 PC（36：3）、PC（36：2）和花生四烯酸（C20：4）与 IM 呈正相关（P<0.05）。醚脂 PCo（38：6）和多种胆甾醇酯与 IM 呈负相关（P<0.05）。ROC 分析表明，花生四烯酸和二十二烷酸（C22：0）可能是区分高 IM 和低 IM 的极好生物标志物。与发现不孕的小母猪类似，初乳摄入不足动物的脂质谱中具有更高丰度的非常长链脂肪酸、不饱和程度更高的脂质和胆甾醇酯，这些脂质在过氧化物酶体中代谢，表明它们可能存在功能障碍。
