Stem Cell Biology Group, Waghmare Lab, Cancer Research Institute, Advanced Centre for Treatment Research and Education in Cancer (ACTREC), Tata Memorial Centre, Kharghar, Navi Mumbai 410210, Maharashtra, India; Homi Bhabha National Institute, Training School Complex, Anushakti Nagar, Mumbai 400085, India.
Stem Cell Biology Group, Waghmare Lab, Cancer Research Institute, Advanced Centre for Treatment Research and Education in Cancer (ACTREC), Tata Memorial Centre, Kharghar, Navi Mumbai 410210, Maharashtra, India.
Biochem Biophys Res Commun. 2023 Oct 15;677:98-104. doi: 10.1016/j.bbrc.2023.07.057. Epub 2023 Aug 4.
Breast cancer is the second most cancer worldwide in females. The primary factor responsible for tumor recurrence is the presence of breast cancer stem cells (BCSCs), which escape the chemo-radiotherapy. In this study, we have investigated the role of Secretory phospholipase-A2 Group 2A (sPLA-IIA) that is overexpressed in BCSCs of MCF7 and MDA-MB-231 breast cancer cell lines. Further, overexpression of sPLA-IIA revealed an increased EGFR/JNK/c-JUN/c-FOS signaling in BCSCs, while sPLA-IIA knockdown significantly reduced the percentage of BCSCs and decreased signaling in both the cell lines. Importantly, sPLA-IIA knockdown showed differentiation of BCSCs. Strikingly, PET imaging showed a decreased metastatic potential of BCSCs. Our study revealed a novel role of sPLA-IIA in regulating BCSCs, which play a crucial role in regulating the differentiation and metastatic potential of BCSCs.
乳腺癌是女性中全球第二大常见癌症。肿瘤复发的主要原因是乳腺癌干细胞（BCSCs）的存在，这些细胞逃避了化疗和放疗。在这项研究中，我们研究了在 MCF7 和 MDA-MB-231 乳腺癌细胞系的 BCSCs 中过度表达的分泌型磷脂酶 A2 组 2A（sPLA-IIA）的作用。此外，sPLA-IIA 的过表达显示在 BCSCs 中 EGFR/JNK/c-JUN/c-FOS 信号的增加，而 sPLA-IIA 的敲低显著降低了 BCSCs 的百分比，并降低了两种细胞系中的信号。重要的是，sPLA-IIA 的敲低显示出 BCSCs 的分化。引人注目的是，PET 成像显示 BCSCs 的转移潜力降低。我们的研究揭示了 sPLA-IIA 在调节 BCSCs 中的新作用，这在调节 BCSCs 的分化和转移潜力方面起着关键作用。
