Mehta Darshan, Paradkar Akshay, Nayak Prakash, Rekhi Bharat, Mohanty Bhabani, Chaudhari Pradip, Waghmare Sanjeev K
Stem Cell Biology Group, Waghmare Lab, Cancer Research Institute, Advanced Centre for Treatment Research and Education in Cancer (ACTREC), Tata Memorial Centre, Kharghar, Navi Mumbai, Maharashtra, 410210, India.
Homi Bhabha National Institute, Training School Complex, Anushakti Nagar, Mumbai, 400085, India.
Hum Cell. 2025 May 25;38(4):108. doi: 10.1007/s13577-025-01237-4.
Cutaneous squamous cell carcinoma (CSCC) is the second most prevalent skin cancer with low metastatic potential; it poses significant morbidity challenges. CSCC possesses significant heterogeneity and the treatment presents a formidable challenge. To gain a clear insight into the diverse nature of these tumors, the development of an in vitro cell line model is essential. However, there are few cell lines that were established, and only one skin SCC cell line is available on the ATCC. In the present study, we established and characterized a novel ACSCC1 cell line from the advanced-stage treatment naïve cutaneous SCC originating from the forearm of the Indian patient. The keratin expression profile showed the epithelial origin of the cell line, ploidy and karyotyping revealed the hyperdiploid population; ACSCC1 showed an increased tumorigenic and metastatic potential. Further, our cell line showed higher invasive, migratory potential and epithelial-mesenchymal-transition (EMT). Additionally, the Transmission electron microscopy (TEM) results showed an aberrant mitochondrial morphology and reduction in the cellular junctions. Further, our whole genome sequencing (WGS) analysis showed mutations in the cancer-related genes. Overall, the novel ACSCC1 cell line can be used to decipher the molecular signaling in the cancer stem cells (CSCs); targeting the CSCs population may help in understanding the tumor recurrence.
皮肤鳞状细胞癌（CSCC）是第二常见的皮肤癌，转移潜力低；它带来了重大的发病挑战。CSCC具有显著的异质性，其治疗面临巨大挑战。为了深入了解这些肿瘤的多样性本质，建立体外细胞系模型至关重要。然而，已建立的细胞系很少，且美国典型培养物保藏中心（ATCC）仅有一种皮肤鳞状细胞癌细胞系。在本研究中，我们从一名印度患者前臂的晚期未经治疗的皮肤鳞状细胞癌中建立并鉴定了一种新型ACSCC1细胞系。角蛋白表达谱显示了该细胞系的上皮来源，倍性和核型分析揭示了超二倍体群体；ACSCC1表现出增加的致瘤和转移潜力。此外，我们的细胞系显示出更高的侵袭、迁移潜力以及上皮-间质转化（EMT）。另外，透射电子显微镜（TEM）结果显示线粒体形态异常且细胞连接减少。此外，我们的全基因组测序（WGS）分析显示癌症相关基因存在突变。总体而言，新型ACSCC1细胞系可用于解读癌症干细胞（CSCs）中的分子信号；靶向CSCs群体可能有助于理解肿瘤复发。
