Zhang Zhuoqi, Shan Jianhua, Shi Bendong, Dong Bowen, Wu Qiong, Zhang Ziwei
College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China.
Animal Science and Technology College, Beijing University of Agriculture, Beijing 100096, PR China.
Ecotoxicol Environ Saf. 2023 Aug 9;262:115336. doi: 10.1016/j.ecoenv.2023.115336.
As environmental pollutants, polybrominated diphenyl ethers (PBDEs) can have toxic effects on living organisms and has a bioaccumulative effect. Low doses of selenium nanoparticles (SeNPs) can exert antioxidant, anti-inflammatory and anti-toxin functions on the organism. This experiment evaluated SeNPs' ability to prevent chicken's intestinal damage from decabromodiphenyl ether (BDE-209) exposure. Sixty layer chickens were separated into four groups at randomly and equally: Control group, SeNPs group (1 mg/kg SeNPs), BDE-209 group (400 mg/kg BDE-209), and BDE-209 +SeNPs group (400 mg/kg BDE-209 and 1 mg/kg SeNPs), for 42 days. The results showed that BDE-209 increased MDA content, decreased the activities of T-SOD, T-AOC, GSH and iNOS, up-regulated the expression of TNF-α, RIPK1, RIPK3 and MLKL, promoted the production of inflammatory factors, reduced the levels of tight junction proteins (Claudin-1, Occludin, ZO-1). SeNPs attenuated intestinal oxidative stress, necroptosis, inflammation and intestinal barrier damage caused by BDE-209. This protective effect is associated with the MAPK/NF-κB signaling pathway. Moreover, SeNPs restores flora alpha and beta diversity, improves intestinal flora composition and its abundance. It shifts the dysbiosis of intestinal flora caused by BDE-209 to normal. Overall, SeNPs can alleviate BDE-209-induced intestinal barrier damage and intestinal flora disorders, which are associated with intestinal oxidative stress, necroptosis and inflammation.
作为环境污染物，多溴二苯醚（PBDEs）可对生物产生毒性作用并具有生物累积效应。低剂量的硒纳米颗粒（SeNPs）可对机体发挥抗氧化、抗炎和抗毒素功能。本实验评估了SeNPs预防十溴二苯醚（BDE - 209）暴露对鸡肠道损伤的能力。将60只蛋鸡随机等分为四组：对照组、SeNPs组（1 mg/kg SeNPs）、BDE - 209组（400 mg/kg BDE - 209）和BDE - 209 + SeNPs组（400 mg/kg BDE - 209和1 mg/kg SeNPs），为期42天。结果表明，BDE - 209增加了丙二醛（MDA）含量，降低了总超氧化物歧化酶（T - SOD）、总抗氧化能力（T - AOC）、谷胱甘肽（GSH）和诱导型一氧化氮合酶（iNOS）的活性，上调了肿瘤坏死因子-α（TNF - α）、受体相互作用蛋白激酶1（RIPK1）、受体相互作用蛋白激酶3（RIPK3）和混合谱系激酶结构域样蛋白（MLKL）的表达，促进了炎症因子的产生，降低了紧密连接蛋白（Claudin - 1、Occludin、ZO - 1）的水平。SeNPs减轻了BDE - 209引起的肠道氧化应激、坏死性凋亡、炎症和肠道屏障损伤。这种保护作用与丝裂原活化蛋白激酶/核因子κB（MAPK/NF - κB）信号通路有关。此外，SeNPs恢复了菌群的α和β多样性，改善了肠道菌群组成及其丰度。它将BDE - 209引起的肠道菌群失调转变为正常状态。总体而言，SeNPs可减轻BDE - 209诱导的肠道屏障损伤和肠道菌群紊乱，这与肠道氧化应激、坏死性凋亡和炎症有关。
