The Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 Youyixi Road Xi'an, Shaanxi, 710072, China.
Biol Trace Elem Res. 2023 Sep;201(9):4484-4496. doi: 10.1007/s12011-022-03513-y. Epub 2022 Dec 5.
Exposure to hypobaric hypoxia at high altitude will cause different tissue and organ damage over a long period of time. Studies have shown that hypobaric hypoxia can cause severe primary intestinal barrier dysfunction, and then cause multiple organ dysfunction. Our previous research showed that selenium nanoparticles (SeNPs) synthesized by Lactobacillus casei ATCC 393 (L. casei ATCC 393) can effectively alleviate intestinal barrier dysfunction caused by oxidative stress and inflammation in mice. This study was conducted to investigate the protective effect of biological SeNPs synthesized by L. casei ATCC 393 on intestinal barrier function in acute hypobaric hypoxic stress mice. The results showed that compared with the hypobaric hypoxic, the SeNPs synthesized by L. casei ATCC 393 by oral administration could effectively alleviate the shortening of intestinal villi, which decreased the level of diamine oxidase (DAO) and myeloperoxidase (MPO), and the expression level of tight junction protein in ileum was increased. In addition, SeNPs significantly increased the activities of superoxide dismutase (SOD), cyclooxygenase (COX-1) and glutathione peroxidase (GPx), and decreased the level of malondialdehyde (MDA), and inhibit the increase of hypoxia related factor. SeNPs effectively regulate the intestinal microecology disorder caused by hypobaric hypoxia stress, and maintain the intestinal microecology balance. In addition, oral administration of SeNPs had better protective effect on intestinal barrier function of mice under hypobaric hypoxia stress. These results suggested that SeNPs synthesized by L. casei ATCC 393 can effectively alleviate the damage of intestinal barrier function under acute hypobaric hypoxic stress, which may be closely related to the antioxidant activity of SeNPs.
在高海拔地区暴露于低氧环境会导致长时间的不同组织和器官损伤。研究表明，低氧环境会导致严重的原发性肠道屏障功能障碍，进而导致多器官功能障碍。我们之前的研究表明，由干酪乳杆菌 ATCC393（L. casei ATCC393）合成的硒纳米颗粒（SeNPs）可以有效缓解氧化应激和炎症引起的小鼠肠道屏障功能障碍。本研究旨在探讨由 L. casei ATCC393 合成的生物 SeNPs 对急性低氧应激小鼠肠道屏障功能的保护作用。结果表明，与低氧环境相比，通过口服给予由 L. casei ATCC393 合成的 SeNPs 可以有效缓解肠绒毛缩短，降低二胺氧化酶（DAO）和髓过氧化物酶（MPO）水平，增加回肠紧密连接蛋白的表达水平。此外，SeNPs 显著增加超氧化物歧化酶（SOD）、环氧化酶（COX-1）和谷胱甘肽过氧化物酶（GPx）的活性，降低丙二醛（MDA）水平，并抑制缺氧相关因子的增加。SeNPs 有效调节低氧应激引起的肠道微生态紊乱，维持肠道微生态平衡。此外，口服 SeNPs 对低氧应激小鼠肠道屏障功能具有更好的保护作用。这些结果表明，由 L. casei ATCC393 合成的 SeNPs 可有效缓解急性低氧应激引起的肠道屏障功能损伤，这可能与 SeNPs 的抗氧化活性密切相关。
