定量光致荧光可有效检测不同环境中的正畸粘接剂残留。

Quantitative light-induced fluorescence enables effective detection of orthodontic adhesive residues in diverse environments.

机构信息

Department of Orthodontics, Yonsei University College of Dentistry, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea; Institute of Craniofacial Deformity, Yonsei University College of Dentistry, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea.

Institute of Craniofacial Deformity, Yonsei University College of Dentistry, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea; BK21 FOUR Project, Yonsei University College of Dentistry, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea.

出版信息

Photodiagnosis Photodyn Ther. 2023 Dec;44:103743. doi: 10.1016/j.pdpdt.2023.103743. Epub 2023 Aug 9.

DOI:10.1016/j.pdpdt.2023.103743

PMID:37567330

Abstract

BACKGROUND

Adhesive remnants post-orthodontic treatment might have deleterious effects on oral health, including enamel demineralization, plaque accumulation, and elevated risk of caries development. The aim of this study was to identify and characterize adhesive residues in an ex vivo environment rich in salivary microbiota using quantitative light-induced fluorescence (QLF) technology.

METHODS

Disc-shaped adhesive samples with thickness ranging from 800 to 100 µm were prepared using GC Ortho, GOTO, T Orthobond, and Transbond XT and subsequently evaluated utilizing a QLF system. Bovine teeth containing GC Ortho and GOTO adhesives and isolated human premolar teeth bonded with brackets were subjected to a 10-day incubation in an artificial saliva environment. Daily imaging was conducted using QLF during incubation. Data with ΔR > 30% and simple hygiene score (SHS) were obtained with a software for further analysis.

RESULTS

Fluorescence intensity exhibited significant differences among the four orthodontic adhesives (p < 0.05). Results of incubation in artificial saliva revealed that red fluorescence surrounding the adhesive on the tooth surface was distinctly observable from day five onwards, with ΔR > 30% and SHS levels higher than those of the control group without adhesive (p < 0.05). Observation of fluorescence images of isolated human premolar teeth with bonded brackets indicated that red fluorescence was primarily present around the brackets.

CONCLUSIONS

Application of QLF is efficacious in identifying and demarcating adhesive residues within an environment rich in salivary microbiota.

摘要

背景

正畸治疗后的黏附残余物可能对口腔健康产生有害影响，包括釉质脱矿、菌斑积聚和龋齿发展风险升高。本研究旨在使用定量光诱导荧光 (QLF) 技术在富含唾液微生物群的体外环境中识别和描述黏附残余物。

方法

使用 GC Ortho、GOTO、T Orthobond 和 Transbond XT 制备厚度为 800-100 µm 的圆盘状黏附样本，随后使用 QLF 系统进行评估。含有 GC Ortho 和 GOTO 黏附剂的牛牙和用托槽黏附的离体人前磨牙在人工唾液环境中孵育 10 天。在孵育过程中，每天使用 QLF 进行成像。使用软件获得 ΔR > 30% 和简单卫生评分 (SHS) 的数据以进行进一步分析。