Key Laboratory of Jianghuai Agricultural Product Fine Processing and Resource Utilization of Ministry of Agriculture and Rural Affairs, Department of Food Science and Engineering, Anhui Agricultural University, Hefei 230036, China.
Key Laboratory of Jianghuai Agricultural Product Fine Processing and Resource Utilization of Ministry of Agriculture and Rural Affairs, Department of Food Science and Engineering, Anhui Agricultural University, Hefei 230036, China.
Carbohydr Polym. 2023 Nov 1;319:121181. doi: 10.1016/j.carbpol.2023.121181. Epub 2023 Jul 14.
The current serious environmental problems have greatly encouraged the design and development of food packaging materials with environmental protection, green, and safety. This study aims to explore the synergistic effect and corresponding mechanism of cellulose nanocrystals (CNC) and CaCl to enhance the film-forming properties of pea protein isolate (PPI). The combination of 0.5 % CNC and 4.5 mM CaCl resulted in a 76.6 % increase in tensile strength when compared with pure PPI-based film. Meanwhile, this combination effectively improved the barrier performance, surface hydrophobicity, water resistance, and biodegradability of PPI-based film. The greater crystallinity, viscoelasticity, lower water mobility, and improved protein spatial conformation were also observed in CNC/CaCl composite film. Compared with the control, the main degradation temperature of composite film was increased from 326.23 °C to 335.43 °C. The CNC chains bonded with amino acid residue of pea protein at specific sites via non-covalent forces (e.g., hydrogen bonds, Van der Waals forces). Meanwhile, Ca promoted the ordered protein aggregation at suitable rate and degree, accompanied by the formation of more disulfide bonds. Furthermore, proper Ca could strengthen the cross-linking and interaction between CNC and protein, thereby establishing a stable network structure. The prepared composite films are expected to be used for strawberry preservation.
当前严峻的环境问题极大地鼓励了设计和开发具有环保、绿色和安全的食品包装材料。本研究旨在探索纤维素纳米晶体（CNC）和 CaCl 的协同作用及其相应的机制，以增强豌豆分离蛋白（PPI）成膜性能。当 0.5%的 CNC 和 4.5mM 的 CaCl 组合使用时，与纯 PPI 基膜相比，拉伸强度增加了 76.6%。同时，这种组合有效地提高了 PPI 基膜的阻隔性能、表面疏水性、耐水性和生物降解性。在 CNC/CaCl 复合膜中还观察到更大的结晶度、粘弹性、更低的水分迁移率和改善的蛋白质空间构象。与对照组相比，复合膜的主要降解温度从 326.23°C 增加到 335.43°C。CNC 链通过非共价力（例如氢键、范德华力）与豌豆蛋白的氨基酸残基结合。同时，Ca 以合适的速率和程度促进有序的蛋白质聚集，伴随着形成更多的二硫键。此外，适量的 Ca 可以增强 CNC 和蛋白质之间的交联和相互作用，从而建立稳定的网络结构。所制备的复合膜有望用于草莓保鲜。
