Key Laboratory of Jianghuai Agricultural Product Fine Processing and Resource Utilization of Ministry of Agriculture and Rural Affairs, State Key Laboratory of Tea Plant Biology and Utilization, School of Tea and Food Science & Technology, Anhui Agricultural University, Hefei 230036, China.
Anhui Bi Lv Chun Biotechnology Co., Ltd., Chuzhou 239200, China.
Carbohydr Polym. 2024 Nov 1;343:122481. doi: 10.1016/j.carbpol.2024.122481. Epub 2024 Jul 10.
The low solubility of pea protein isolate (PPI) greatly limits its functional properties and its wide application in food field. Thus, this study investigated the effects and mechanisms of cellulose nanocrystals (CNC) (0.1-0.4 %) and CaCl (0.4-1.6 mM) on the solubility of PPI. The results showed that the synergistic effect of CNC (0.3 %) and Ca (1.2 mM) increased the solubility of PPI by 242.31 %. CNC and Ca changed the molecular conformation of PPI, enhanced intermolecular forces, and thus induced changes in the molecular morphology of PPI. Meanwhile, the turbidity of PPI decreased, while surface hydrophobicity, the absolute zeta potential value, viscoelasticity, β-sheet ratio, and thermal properties increased. CNC bound to PPI molecules through van der Waals force and hydrogen bond. Ca could strengthen the crosslinking between CNC and PPI. In summary, it is proposed a valuable combination method to improve the solubility of PPI, and it is believed that this research is of great significance for expanding the application fields of PPI and modifying plant proteins.
豌豆分离蛋白（PPI）的低溶解度极大地限制了其功能特性及其在食品领域的广泛应用。因此，本研究探讨了纤维素纳米晶体（CNC）（0.1-0.4%）和 CaCl（0.4-1.6mM）对 PPI 溶解度的影响及其机制。结果表明，CNC（0.3%）和 Ca（1.2mM）的协同作用将 PPI 的溶解度提高了 242.31%。CNC 和 Ca 改变了 PPI 的分子构象，增强了分子间作用力，从而导致 PPI 分子形态发生变化。同时，PPI 的浊度降低，表面疏水性、绝对 zeta 电位值、粘弹性、β-折叠比例和热性能增加。CNC 通过范德华力和氢键与 PPI 分子结合。Ca 可以加强 CNC 和 PPI 之间的交联。总之，提出了一种提高 PPI 溶解度的有效组合方法，相信本研究对扩大 PPI 的应用领域和修饰植物蛋白具有重要意义。
