Ethiopian Ministry of Agriculture, Food and Nutrition Office, Food Safety and Quality Desk, P. O. Box. 62347, Addis Ababa, Ethiopia.
Food Hygiene and Technology Department, Faculty of Veterinary Medicine, Near East University, 99138, Nicosia, Cyprus.
Sci Rep. 2023 Aug 11;13(1):13081. doi: 10.1038/s41598-023-40260-z.
Spirulina platensis has a wide range of activities, notably antibacterial property against food pathogens. This study investigates the antibacterial activity of S. platensis extract on Total Mesophilic and Psychrophilic Aerobic Bacteria. The results were compared using statistical analysis and the predicted model values using artificial intelligence-based models such as artificial neural network (ANN) and adaptive neuro fuzzy inference system (ANFIS) Models. The extraction of spirulina was done by using the freeze-thaw method with a concentration of 0.5, 1 and 5% w/v. Before the application of the extract, initial microbial load of fillets was analyzed the and the results were used as control. After application analysis was performed at 1, 24 and 48 h of storage at 4 °C. Based on the statistical analysis result the S. platensis extracts' antimicrobial activity over TMAB of fresh tilapia fish fillets at 1, 24 and 48 h was using EA from 2.5 log10 CFU/g during the control stage to 1.8, 1.1 and 0.7 log10 CFU/g respectively whereas EB and EC was from 2.1 and 2.2 log10 CFU/g at control to 1.5, 0.8, 0.5 log10 CFU/g and 1.23, 0.6 and 0.32 log10 CFU/g respectively at the specified hour interval. Similarly, the three extracts over TPAB were from 2.8 log10 CFU/g at control time to 2.1, 1.5 and 0.9 in EA, while using EB reduces from 2.8 log10 CFU/g to 1.9, 1.3 and 0.8 log10 CFU/g at 1, 24 and 48 h respectively. Although EC presented the reduction from 1.9 log10 CFU/g to 1.4, 1 and 0.5 log10 CFU/g. This was supported by ANN and ANFIS models prediction.
螺旋藻具有广泛的活性，特别是对食源性病原体具有抗菌特性。本研究调查了螺旋藻提取物对总需氧和嗜冷好氧细菌的抗菌活性。使用人工神经网络 (ANN) 和自适应神经模糊推理系统 (ANFIS) 等基于人工智能的模型对结果进行了比较，并预测了模型值。使用 0.5、1 和 5% w/v 的浓度通过冻融法提取螺旋藻。在应用提取物之前，分析了鱼片的初始微生物负荷，并将结果用作对照。在 4°C 下储存 1、24 和 48 小时后进行应用分析。根据统计分析结果，在 1、24 和 48 小时的新鲜罗非鱼片的 TMAB 中，螺旋藻提取物对新鲜罗非鱼片的抗菌活性使用 EA 从对照阶段的 2.5 log10 CFU/g 分别降至 1.8、1.1 和 0.7 log10 CFU/g，而 EB 和 EC 从 2.1 和 2.2 log10 CFU/g 分别降至 1.5、0.8 和 0.5 log10 CFU/g 和 1.23、0.6 和 0.32 log10 CFU/g 分别在指定的小时间隔内。同样，三种提取物在 TPAB 中从对照时间的 2.8 log10 CFU/g 分别降至 EA 中的 2.1、1.5 和 0.9，而使用 EB 从 2.8 log10 CFU/g 分别降至 1.9、1.3 和 0.8 log10 CFU/g 分别在 1、24 和 48 小时。尽管 EC 从 1.9 log10 CFU/g 分别降至 1.4、1 和 0.5 log10 CFU/g，但这得到了 ANN 和 ANFIS 模型预测的支持。
