Perrone Alina E, Pinillo Mariana, Rial Marcela S, Fernández Marisa, Milduberger Natalia, González Carolina, Bustos Patricia L, Fichera Laura E, Laucella Susana A, Albareda María Cecilia, Bua Jacqueline
Instituto Nacional de Parasitología Dr. Mario Fatala Chaben-ANLIS-Malbrán, Av. Paseo Colón 568, Buenos Aires 1063, Argentina.
Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires 1063, Argentina.
Int J Mol Sci. 2023 Jul 25;24(15):11875. doi: 10.3390/ijms241511875.
Cyclophilins (CyPs) are a family of enzymes involved in protein folding. , the causative agent of Chagas disease, has a 19-kDa cyclophilin, CyP19, that was found to be secreted in parasite stages of the CL Brener clone and recognized by sera from -infected mice and patients. The levels of specific antibodies against CyP19 in -infected mice and subjects before and after drug treatment were measured by an in-house enzyme linked immunosorbent assay (ELISA). Mice in the acute and chronic phase of infection, with successful trypanocidal treatments, showed significantly lower anti-CyP19 antibody levels than untreated mice. In children and adults chronically infected with , a significant decrease in the anti-CyP19 titers was observed after 12 months of etiological treatment. This decrease was maintained in adult chronic patients followed-up 30-38 months post-treatment. These results encourage further studies on CyP19 as an early biomarker of trypanocidal treatment efficiency.
亲环蛋白（CyPs）是一族参与蛋白质折叠的酶。恰加斯病的病原体克氏锥虫有一个19 kDa的亲环蛋白，即CyP19，它被发现存在于CL Brener克隆株的寄生虫阶段，并能被来自克氏锥虫感染小鼠和患者的血清识别。通过一种内部酶联免疫吸附测定（ELISA）来检测克氏锥虫感染小鼠和受试者在药物治疗前后针对CyP19的特异性抗体水平。处于感染急性期和慢性期且接受了成功杀锥虫治疗后的小鼠，其抗CyP19抗体水平显著低于未治疗的小鼠。在慢性感染克氏锥虫的儿童和成人中，病因治疗12个月后观察到抗CyP19滴度显著下降。这种下降在治疗后30 - 38个月进行随访的成年慢性患者中持续存在。这些结果促使人们进一步研究CyP19作为杀锥虫治疗效果早期生物标志物的作用。
